作 者：
刘兰香;伊冠东;李凯;马金菊;徐涓;孙彦琳;张弘
单 位：
昆明理工大学化学工程学院;中国林业科学研究院资源昆虫研究所
摘 要：
研究溶剂、浓度、pH值以及金属离子对胭脂虫红酸荧光性能的影响,表明胭脂虫红酸在pH 4且其物质的量浓度为2.0×10~(-4) mol/L的水溶液中荧光最强;而胭脂虫红酸对金属离子选择性识别的结果显示,Al~(3+)能使胭脂虫红酸的荧光强度显著增强从而提高检测的灵敏度。以Al~(3+)(2.0×10~(-3) mol/L)为标准溶液检测样品中胭脂虫红酸含量时,胭脂虫红酸的物质的量浓度在1.0×10~(-5)～8.0×10~(-5) mol/L范围内与其荧光强度呈良好的线性关系,检测限为5.0×10~(-6) mol/L。实际应用结果表明,用荧光法检测不同酸奶和乳酸菌饮料中添加的胭脂虫红酸的含量,样品回收率在96.6%～110.5%之间,相对标准偏差小于5.9%。本研究为快速、有效、定量地检测食品中的胭脂虫红酸提供了一种有效的方法。
译 名：
Rapid Detection of Carminic Acid in Yogurt and Lactobacillus-Containing Beverage by Al~(3+)-Based Fluorescence Enhancement
作 者：
LIU Lanxiang;YI Guandong;LI Kai;MA Jinju;XU Juan;SUN Yanlin;ZHANG Hong;Research Institute of Resources Insects, Chinese Academy of Forestry;Faculty of Chemical Engineering, Kunming University of Science and Technology;
关键词：
carminic acid;;fluorescence;;enhancement;;fast detection
摘 要：
The effects of solvents, carminic acid concentration, pH and metal ions on the fluorescence properties of carminic acid were systematically studied. The fluorescent intensity of carminic acid was the strongest at a concentration of 2.0 × 10~(-4) mol/L in an aqueous solution at pH 4. The selectivity of carminic acid to metal ions indicated that the addition of Al~(3+) could significantly enhance the fluorescence intensity of carminic acid thus improving the sensitivity of detection. A 2.0 × 10~(-3) mol/L aqueous solution of Al~(3+)(at) was used as the standard solution. The.uorescence intensity of carminic acid was linear with its concentration ranging from 1.0×10~(-5) to 8.0×10~(-5) mol/L, and the limit of detection(LOD) was 5.0×10~(-6 )mol/L.When the fluorescence method was applied to determine carminic acid in yogurts and Lactobacillus-containing beverages,the recoveries ranged from 96.6% to 110.5%, relative standard deviation(RSD) no more than 5.9%. This study provides an effective approach to rapidly and quantitatively detect carminic acid in foods.
