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当前位置: 首页 > 文章 > 条斑紫菜6个品系的SRAP分析 水产学报 2013,37 (10) 1495-1502
Position: Home > Articles > Analysis and identification of different strains of Pyropia yezoensis using sequence-related amplified polymorphism markers Journal of Fisheries of China 2013,37 (10) 1495-1502

条斑紫菜6个品系的SRAP分析

作  者：
贾威;黄林彬;严兴洪
单  位：
上海海洋大学水产与生命学院
关键词：
条斑紫菜;相关序列扩增多态性;分子标记;遗传距离;指纹图谱
摘  要：
为鉴别条斑紫菜不同品系的种质,使用相关序列扩增多态性(sequence-related amplified polymorphism,SRAP)标记对条斑紫菜的5个选育品系和1个野生品系进行遗传分析,结果从35对引物组合中筛选出可扩增出稳定清晰条带的组合11对,共获得131个扩增位点,其中多态性位点125个,多态性比例高达95.42%。6个品系间的遗传距离为0.364 3~0.867 9,平均为0.593 0。用UPGMA法进行聚类分析,结果将6个品系分为2个群,所反映的亲缘关系与各品系的来源基本一致,说明SRAP标记技术可以成为条斑紫菜品系间遗传分析的有效工具。在131个多态性位点中,选择扩增出的4个位点构建了6个品系的指纹图谱。另外,通过ME1/EM6引物组合扩增得到耐高温品系TM-18的特异性条带,经回收测序和重新设计引物,该条带在其丝状体和叶状体DNA中均能稳定地被扩增出来,可用于该品系的种质鉴别。
译  名：
Analysis and identification of different strains of Pyropia yezoensis using sequence-related amplified polymorphism markers
作  者：
JIA Wei;HUANG Linbin;YAN Xinghong;College of Fisheries and Life Science,Shanghai Ocean University;Key Laboratory of Exploration and Utilization of Aquatic Resources,Shanghai Ocean University;
关键词：
Pyropia yezoensis;;sequence-related amplified polymorphism(SRAP);;molecular marker;;genetic distance;;fingerprints
摘  要：
6 strains of Pyropia yezoensis( Ueda) M. S. Hwang et H. G. Choi were analyzed using SRAP (sequence-related amplified polymorphism) markers in order to identify the germplasm. DNAs from conchocelis of 6 strains were screened with 35 primer combinations,of which 11 primer combinations gave stable and reproducible amplification patterns. Among the total 131 fragments,125 fragments( 95.42%) were polymorphic. The genetic distances of 6 strains were between 0.3643 and 0.8679,and the average was 0.5930. Cluster analysis of UPMGA showed a good and true relationships among the 6 strains. The results demonstrated that SRAP could be a useful tool in germplasm identification of P. yezoensis strains. From the total 131 fragments,4 fragments amplified by 1 primer combination were used to develop the DNA fingerprints of 6 strains of P. yezoensis. One specific fragment of TM-18 was obtained in the fragments amplified by primer combination ME1/EM6. According to the DNA sequence of the fragments,a specific pair-primer was designed and it achieved stable amplification of a 340bp specific band in both DNA of conchocelis and blades of TM-18. The specific marker could be used in fast identification of TM-18 strain.

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