单 位：
河北农业大学动物医学院;井陉县动物卫生监督所
摘 要：
为优化自生地黄中同时提取地黄多糖和梓醇的工艺,本研究采用单因素试验和正交试验进行优选,用苯酚硫酸法测定地黄多糖的含量和高效液相色谱法测定梓醇的含量,以地黄多糖和梓醇的得率为评价指标,考察固液比、提取时间和提取温度对得率的影响。最终确定最佳工艺参数为固液比1∶8,提取时间为1.5h,提取温度为90℃。该工艺稳定可行,适合工业化生产,可为新兽药开发利用提供参考。
译 名：
Extraction Technology of Rehmannia glutinosa Polysaccharide and Catalpol from Radix Rehmanniae Simultaneously
作 者：
SUN Yue-chuan;ZHENG Hua;LIU Jing;WANG Lin;LIU Ju-xiang;College of Veterinary Medicine,Agricultural University of Hebei;Animal Health Inspection of Jingxing;
关键词：
Rehmannia glutinosa polysaccharide;;catalpol;;extraction technology
摘 要：
To optimize the extraction technology of Rehmannia glutinosa polysaccharide and catalpol fromRadix Rehmanniae simultaneously,a single factor test and orthogonal test were used in this experiment.Content of Rehmannia glutinosa polysaccharides and catalpol were determined by phenol-sulfuric acid method and HPLC,respectively.The yields of Rehmannia glutinosa polysaccharide and catalpol were used as the evaluating parameters,and the effects of the solid to liquid ratio,extraction temperature and extraction time on the yield were examined.The optimum extraction technological parameters were as follows:solid to liquid ratio was 1∶8,the extraction time was 1.5h,and the extraction temperature was 90℃.This technique was stable and feasible,and was appropriate for industrial production,and would provide references for the development and utilization of new veterinary drugs.
