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当前位置: 首页 > 文章 > 甘肃冬小麦品种(系)面筋强度、黄色素含量和PPO活性相关基因的分子检测 甘肃农业大学学报 2015 (3) 54-60
Position: Home > Articles > Molecular detection of genes associated with gluten strength,yellow pigment content and PPO activity of winter wheat cultivars (lines) in Gansu Province Journal of Gansu Agricultural University 2015 (3) 54-60

甘肃冬小麦品种(系)面筋强度、黄色素含量和PPO活性相关基因的分子检测

作  者：
周喜旺;宋建荣;岳维云;张耀辉;曹世勤;吕莉莉;刘鸿燕;王娜;南海;赵尚文
单  位：
甘肃省天水市农业科学研究所;甘肃省农业科学院植物保护研究所
关键词：
普通小麦;1Dx5;黄色素;多酚氧化酶活性;分子检测
摘  要：
为了明确甘肃冬小麦品种(系)中品质相关基因的分布状况,提高品质育种效率,以141份小麦品种(系)为材料,利用高分子量麦谷蛋白亚基1Dx5的特异PCR标记、与黄色素含量相关的八氢番茄红素合酶(phytoene synthase,Psy)基因Psy-A1的标记YP7A及多酚氧化酶(polyphenol oxidase,PPO)活性基因Ppo-A1的标记PPO18分别进行1 Dx5、Psy-A1和Ppo-A1的基因型检测.结果表明:含1 Dx5基因的材料56份,分布频率为39.7%;Psy-A1位点存在Psy-A1a和Psy-A1b2种等位变异类型,分布频率分别为62.4%和37.6%;Ppo-A1位点存在Ppo-A1a和Ppo-A1b2种等位变异类型,分布频率分别为42.6%和57.4%.1 Dx5、Psy-A1和Ppo-A1的基因类型在不同地区分布频率存在明显差异,1 Dx5和Psy-A1b基因在天水地区频率最高,分别为51.0%和40.8%;Ppo-A1b基因在庆阳地区频率最高,为77.8%,Psy-A1a基因在3地区的分布频率明显高于Psy-A1b基因.参试材料中,聚合1 Dx5、Psy-A1b和Ppo-A1b基因的材料有19份,这些材料可作亲本资源,在小麦品种面筋强度和面粉色泽的改良中加以利用.
译  名：
Molecular detection of genes associated with gluten strength,yellow pigment content and PPO activity of winter wheat cultivars (lines) in Gansu Province
作  者：
ZHOU Xi-wang;SONG Jian-rong;YUE Wei-yun;ZHANG Yao-hui;CAO Shi-qin;LYU Li-li;LIU Hong-yan;WANG Na;NAN Hai;ZHAO Shang-wen;Tianshui Institute of Agricultural Sciences;Institute of Plant Protection,Gansu Academy of Agricultural Sciences;
关键词：
common wheat;;1Dx5;;yellow pigment;;polyphenol oxidase activity;;molecular detection
摘  要：
In order to define the distribution of genes related to quality traits and improve breeding efficiency,3gene specific markers for HMW-GS1Dx5,Psy-A1 and Ppo-A1 genes were used to detect the allelic variations at these loci in 141 Gansu wheat cultivars and advanced lines.The results indicated that the frequency of 1Dx5 gene was 39.7%,the frequencies of Psy-A1 agenotype with high yellow pigment content and Psy-A1 bgenotype with low yellow pigment content at Psy-A1 locus were 62.4% and 37.6%,respectively;the frequencies of Ppo-A1 agenotype with high PPO activity and Ppo-A1 bgenotype with low PPOactivity at Ppo-A1 locus were 42.6%and 57.4%,respectively.Significant differences among 1Dx5,Psy-A1 and Ppo-A1 were found in different areas,1Dx5 and Psy-A1 b were distributed mainly in Tianshui area,with frequency of 51.0% and 40.8%,respectively;Ppo-A1 bhad highest frequency of 77.8%in Qingyang area.The frequency of Psy-A1 awas obviously higher than that of Psy-A1 bin three areas.19 materials contented desired gene for 1Dx5 and alleles for Psy-A1 b and Ppo-A1 b,thus it is essential to combine the desired genes in a variety to improve the processing quality of Gansu winter wheat.The markers used in this study were all gene-specific,with good stability and high accuracy,and they can be efficiently applied in molecular marker-assisted breeding for wheat quality improvement.

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