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当前位置: 首页 > 文章 > 梨S_(12)-RNase基因启动子植物表达载体的构建及转基因研究 中南林业科技大学学报 2010,30 (8) 11-17
Position: Home > Articles > Cloning of S_(12)-RNase promoter region of Chinese pear (Pyrus pyrifolia) and construction of plant expression vector Journal of Central South University of Forestry & Technology 2010,30 (8) 11-17

梨S_(12)-RNase基因启动子植物表达载体的构建及转基因研究

作  者：
刘学英;乌云塔娜
单  位：
中南林业科技大学林学院;中南林业科技大学经济林育种与栽培国家林业局重点实验室
关键词：
生物科学与技术;梨属;中国梨;梨S12-RNase基因;启动子;植物表达载体;转基因研究
摘  要：
为了检测S12-RNase启动子的表达特性,以pBI101.2为基础,构建了砂梨S12-RNase基因启动子5’端系列缺失植物表达载体PS12-(0～5)-GUS-pBll01.2,并通过农杆菌介导的Floral Dip法转化哥伦比亚野生型拟南芥。卡那霉素和PCR鉴定表明:GUS基因已整合到转基因植株的基因组中,为下一步进行启动子功能的鉴定奠定了基础。
译  名：
Cloning of S_(12)-RNase promoter region of Chinese pear (Pyrus pyrifolia) and construction of plant expression vector
作  者：
LIU Xue-yinga,WUYUN Ta-naa,b (a.Key Lab of Non-wood Forest Product of Forestry Ministry;b.School of Forestry,Central South University of Forestry and Technology,Changsha 410004,Hunan,China)
关键词：
biological science and technology;Pyrus;Pyrus pyrifolia;S12-RNase;promoter;plant expression vector;transgenic study
摘  要：
To examine the expression style of S12-RNase promoters,full length and a series of 5'-deletion fragments-GUS fusions were constructed successfully and introduced into Agrobacterium tumefaciens strain GV3101 by using electroporation method and then were introduced into Arabidopsis thaliana plants(ecotype Columbia 0) by Agrobacterium mediated Floral Dip transformation method.A detection of transgenic plants was conducted based on 0.5× MS medium containing 50 μg/mL Kanamycin and PCR,and it is founded that the GUS gene had been integrated into the genome of the transgenic plants,and this study has laid a foundation for further identifying the expression and function of the promoters.

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