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Position: Home > Articles > TLC Identification and Content Determination of Quercetin hydropiper in Polygonumin Guizhou Guizhou Agricultural Sciences 2015,43 (10) 191-194+199

黔产辣蓼中槲皮素的薄层鉴别与含量测定

作  者:
刘亮;冯华;刘英波;潘年松;周德权
单  位:
遵义市食品药品检验所;遵义医药高等专科学校;遵义绿普森农业有限公司
关键词:
辣蓼;槲皮素;薄层鉴别;含量测定
摘  要:
为建立黔产辣蓼的薄层鉴别与含量测定方法,采用薄层色谱法,以槲皮素为对照,以甲苯-甲酸乙酯-甲酸(10∶8∶1)为展开剂,建立了其定性鉴别方法;采用高效液相色谱法,以槲皮素为对照,Hypersil ODS2C18(5μm,200mm×4.6mm)为色谱柱,0.20%磷酸溶液-乙腈(80∶20)作为流动相,二极管矩阵检测器为检测器,建立了其含量测定方法。结果表明:建立的TLC法能很好分离各成分,其方法学考察精密度、重复性、稳定性和加样回收率的RSD分别为0.18%、0.71%、1.10%和2.44%,能够准确测定辣蓼中槲皮素的含量。
译  名:
TLC Identification and Content Determination of Quercetin hydropiper in Polygonumin Guizhou
作  者:
LIU Liang;FENG Hua;LIU Ying bo;PAN Linsong;ZHOU Dequan;Zunyi Medical and Pharmaceuticall College;Zunyi Institute of Food & Drug Control;Zunyi Lupusen Agricultural Limited Company;
关键词:
Polygonum hydropiper;;quercetin;;TLC identification;;content determination
摘  要:
The TLC identification method of quercetin is established by using toluene-ethyl formateformic acid(10∶8∶1)as a developing solvent and taking quercetin as CK.The content of quercetin was determined by HPLC under the conditions including chromatographic column of Hypersil ODS2C18(5μm,200mm×4.6mm),moving phase of 0.2% phosphate-acetonitrile(80∶20)and photodiode array detector to establish TLC identification and content determination method of quercetin in P.hydropiper from Guizhou.Results:The established TLC method had a good separation effect on each component.The RSD of accuracy,repeatability,stability and recovery of the established HPLC method is 0.18%,0.71%,1.10%and 2.44%respectively,which can determine the content of quercetin in P.hydropiper accurately.

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