当前位置: 首页 > 文章 > 德国牧羊犬cOR7R1基因的克隆及序列分析 黑龙江畜牧兽医 2013 (3) 137-138
Position: Home > Articles > Cloning and sequence analysis of the cOR7R1 gene in German shepherd dog Heilongjiang Animal Science and Veterinary Medicine 2013 (3) 137-138

德国牧羊犬cOR7R1基因的克隆及序列分析

作  者:
杨敏;李向党
单  位:
公安部沈阳警犬技术学校
关键词:
德国牧羊犬;cOR7R1基因;克隆;序列分析
摘  要:
为了研究德国牧羊犬嗅觉受体基因品种特异性单核苷酸多态性(SNP)位点,试验采用PCR技术对警用德国牧羊犬cOR7R1基因进行扩增、T-A克隆、测序及序列比对分析。结果表明:德国牧羊犬cOR7R1基因与GenBank公布的犬cOR7R1基因遗传同源性为99.8%,存在2处有义突变,即SNP A200G、SNP A868G。
译  名:
Cloning and sequence analysis of the cOR7R1 gene in German shepherd dog
作  者:
YANG Min,LI Xiang-dang(Shenyang Police-dog technology school of the Ministry of Public Security,Shenyang 110034,China)
关键词:
German shepherd dog;cOR7R1 gene;cloning;sequence analysis
摘  要:
To study breed-specific single nucleotide polymorphisms(SNPs) sites of the olfactory receptor gene in German shepherd dog,the cOR7R1 genes for olfactory receptor,which came from German shepherd police dogs,were amplified and cloned through the method PCR and T-A cloning.The recombinant plasmids were sequenced and analyzed by sequence alignment.The results showed the homology was 99.8% compared with the published sequence of dogs from GenBank,and there were two sense mutations,SNP A200G and SNP A868G.

相似文章

计量
文章访问数: 22
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊