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Position: Home > Articles > Effects of gene deletion of katExoo encoding putative catalase on resistance to hydrogen peroxide and pathogenicity of Xanthomonas oryzae pv. oryzae Acta Phytopathologica Sinica 2013 (6) 615-621

katExoo基因缺失突变对水稻白叶枯病菌H_2O_2抗性和致病性的影响

作  者:
王努;吴茂森;田芳;陈华民;何晨阳
单  位:
植物病虫害生物学国家重点实验室;中国农业科学院植物保护研究;中国农业科学院植物保护研究所;所植物病虫害生物学国家重点实验室
关键词:
水稻白叶枯病菌;katExoo;H2O2抗性;致病性
摘  要:
为了揭示过氧化氢酶基因katExoo在水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)过氧化氢(H2O2)抗性和致病性中的功能,本研究构建了基因缺失突变体ΔkatExoo,测定了突变体的H2O2抗性、过氧化氢酶(CAT)活性、在离体培养条件下的生长速率以及对水稻的致病性。用标记基因置换法获得了ΔkatExoo突变体,其保守的CAT结构域(GATase1_catalase和catalase_clade_2)被GmR片段所替换。katExoo基因缺失突变并不导致病菌的H2O2抗性和CAT活性降低或丧失,反而在一定程度上使之增强和升高。ΔkatExoo离体生长量显著降低,水稻接种叶片病斑明显缩短、在叶组织内的种群量下降。表明基因缺失突变显著地影响了病菌的生长、定殖和致病性。本研究结果为"KatExoo可能是Xoo的一个毒性因子"的假设提供了遗传学证据。
译  名:
Effects of gene deletion of katExoo encoding putative catalase on resistance to hydrogen peroxide and pathogenicity of Xanthomonas oryzae pv. oryzae
作  者:
WANG Nu;WU Mao-sen;TIAN Fang;CHEN Hua-min;HE Chen-yang;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;
关键词:
Xanthomonas oryzae pv.oryzae;;katExoo;;H2O2 resistance;;pathogenicity
摘  要:
To reveal the role of katExoo encoding putative catalase( CAT) in H 2 O 2 resistance and pathogenicity of Xanthomonas oryzae pv. oryzae,the gene deletion and assays for H 2 O 2 resistance,CAT activity,in vitro growth,and virulence on rice of the ΔkatExoo mutant were performed in this study. The mutant was generated from Xoo wild-type strain PXO99Athrough marker exchange,in which the conserved domain of CAT( GATase1_catalase and catalase_clade_2) was replaced by GmR. Significant changes in increased H 2 O 2 resistance and higher CAT activity w ere found in the mutant compared to PXO99A. Moreover,remarkable decreases in bacterial grow th rate in vitro and in planta of ΔkatExoo and diseased leaf lesion of rice w ere observed. Therefore,katE- xoo has been show n to play a critical role in bacterial grow th,colonization and pathogenicity on rice. This w ork partially provides first genetic evidence to support the hypothesis that KatExoo might function as a virulence factor in Xoo.

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