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当前位置: 首页 > 文章 > 水稻白叶枯病菌群体感应系统对T3SS基因表达的调控作用分析 植物病理学报 2012,42 (6) 620-625
Position: Home > Articles > Regulation of expression of type Ⅲ secretion system-encoded hrp genes of Xanthomonas oryzae pv. oryzae by diffusible signal factor-mediated quorum sensing Acta Phytopathologica Sinica 2012,42 (6) 620-625

水稻白叶枯病菌群体感应系统对T3SS基因表达的调控作用分析

作  者：
霍欢;孙蕾;田芳;陈华民;何晨阳
单  位：
中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
关键词：
水稻白叶枯病菌;群体感应;DSF;hrp基因;调控
摘  要：
为了阐明水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)利用DSF信号群体感应(QS)系统对毒性表达进行调控的机理,本研究对编码QS的rpf基因进行了分子鉴定,分析了Δrpf基因突变体在水稻叶组织中的种群量变化,利用RT-qPCR方法定量测定了编码T3SS的hrp基因转录本以及rpf基因自身表达。结果表明,rpfF、rpfC和rpfG基因与几种主要植物病原黄单胞菌同源序列高度保守;RpfF是烯脂酰辅酶A水合酶家族成员之一,RpfC含有组氨酸磷酸激酶结构域(His-KA)和磷酸受体结构域(REC),RpfG含有REC和HD-GYP结构域;Δrpf突变体在水稻叶组织中的种群量及其扩展能力均明显下降;hrp基因转录显著地受到QS调控;rpf基因表达与Xoo种群密度密切相关。因此,Xoo QS系统显著地调控了hrp基因的表达,在QS与T3SS表达之间存在一个信号通路。
译  名：
Regulation of expression of type Ⅲ secretion system-encoded hrp genes of Xanthomonas oryzae pv. oryzae by diffusible signal factor-mediated quorum sensing
作  者：
HUO Huan,SUN Lei,TIAN Fang,CHEN Hua-min,HE Chen-yang(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
关键词：
Xanthomonas oryzae pv.oryzae;quorum sensing;diffusible signal factor;hrp genes;regulation
摘  要：
Bacterial virulence of Xanthomonas oryzae pv.oryzae(Xoo) was previously shown to be regulated by the diffusible signal factor(DSF)-mediated quorum sensing(QS).To further elucidate the regulatory mechanisms,structural features of QS-encoded rpf genes was identified through the bioinfomatic analysis,bacterial population of Δrpf mutants in the infacted leaf tissues of rice was detected,and transcription leavels of type Ⅲ secretion system(T3SS)-encoded hrp genes were measured via RT-qPCR assays in this study.The results showed that rpf genes were found to be highly conserved in plant-pathogenic Xanthomonas spp.RpfF was one of enoyl-CoA hydratase family members,RpfC was structurally featured in N-terminal histidine kinase domain and C-terminal receiver(REC) domain,while RpfG contained REC and HD-GYP domains.Significant decreases in bacterial population and spread in the rice leaf tissues of Δrpf mutants were observed compared with wild-type strain PXO99A.Transcription of hrp genes was significantly regulated by QS.Expression of rpf genes was regulated by Rpf proteins and bacterial density-dependent.Therefore,it might be suggested that QS regulated T3SS expression via the DSF/c-di-GMP-mediated signaling pathway(s).

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