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中国黄牛重组PrP~c成熟蛋白单克隆抗体的研制

作  者:
吴晓东;王志亮
单  位:
农业部动物检疫所全国疯牛病检测中心
关键词:
中国黄牛;重组PrPc成熟蛋白;单克隆抗体
摘  要:
将纯化的牛重组PrPc 成熟蛋白免疫PrPc 基因敲除小鼠 (PrPc-nullmice) ,利用淋巴细胞杂交瘤技术 ,经一次融合 ,共筛选出3株能稳定分泌针对中国黄牛PrPc 成熟蛋白特异单克隆抗体的杂交瘤细胞株4D5、5C10和3F3。免疫印迹试验表明 :腹水单抗4D5、5C10和3F3均可特异识别重组中国黄牛PrPc 成熟蛋白
译  名:
Development of Monoclonal Antibody Specific to recombinant PrP~c Mature Protein of Chinese Yellow Cattle
作  者:
Wu XiaoDong Wang ZhiLiang (Animla Quarantine Inslitute,MOA Qingdao 266032 China)
关键词:
Chinese yellow cattle Recombinant PrPc mature protein Mono clonal antibody
摘  要:
Three hybridoma cell lines (4D5, 5C10 and 3F3),stably secreting monoclonal antibodies specific to PrPc mature protein of Chinese yellow cattle, were selected by immunization of PrPc-null mice with purified recombinant bovine PrP mature protein and by means of lymphocyte hybridoma technique. Western blotting showed that the monoclonal antibodies 4D5, 5C10 and 3F3 could specifically recognize the recombinant PrPc mature protein of Chinese yellow cattle.

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