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Position: Home > Articles > Analysis of Sequences of BoLA-DRB_3 Genes in Chinese Yellow Cattle Chinese Journal of Animal and Veterinary Sciences 2008,39 (7) 853-857

部分中国黄牛BoLA-DRB_3基因序列分析

作  者:
王昆;张沅;孙东晓
单  位:
河北省农林科学院粮油作物研究所;中国农业大学动物科技学院
关键词:
中国黄牛;BoLA-DRB3外显子2;等位基因;序列测定;多态性
摘  要:
通过DNA测序,分别对4个中国黄牛地方品种的MHC-Ⅱ类基因DRB3外显子2的多态性进行分析。通过序列比对,共检测到DRB3*exon2等位基因259种,其中新等位基因131个。等位基因的分布具有品种特异性。各等位基因之间存在大量多态位点,统计分析表明:发生非同义替换的多态位点均主要分布在抗原结合位点对应的编码序列中,共有17个变异位点的氨基酸组成在品种间具有显著差异,其中7个位点差异显著(P<0.05);10个位点差异极显著(P<0.01)。在变异位点中,有8个位点为抗原结合位点。
译  名:
Analysis of Sequences of BoLA-DRB_3 Genes in Chinese Yellow Cattle
作  者:
WANG Kun1,ZHANG Yuan2,SUN Dong-xiao2(1.Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050031,China;2.College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)
关键词:
Chinese Yellow cattle;BoLA-DRB3* exon2;allele;sequencing;polymorphism
摘  要:
In this research,four Chinese Yellow cattle breeds were studied for the molecular genetic diversity in MHC-DRB3*exon2 by DNA sequencing.A total of 259 DRB3*exon2 alleles including 131 newly identified ones were found.The number of alleles represents differences in breeds,indicating genetic variations among different breeds.The estimation of the average rate of synonymous(dS)and non-synonymous(dN)substitution in the exon 2 of BoLA-DRB3 showed there was a higher ratio of dN to dS substitutions in the peptide-binding site(PBS) region.The amino acid components of 17 polymorphic sites were significant difference among breeds,including 8 PBS.

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