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当前位置: 首页 > 文章 > 高效液相色谱测定紧压茶中有机酸条件研究 茶叶 2005,31 (4) 224-227
Position: Home > Articles > Simultaneous Determination of Various Organic Acids in Compressed Tea by High Performance Liquid Chromatography Journal of Tea 2005,31 (4) 224-227

高效液相色谱测定紧压茶中有机酸条件研究

作  者：
丁玲;屠幼英;陈晓敏
单  位：
浙江大学茶学系
关键词：
高效液相色谱;测定;有机酸;紧压茶
摘  要：
建立一种利用高效液相色谱测定紧压茶中10种有机酸的方法。紧压茶中有机酸的色谱条件为:Di-amonsilC18柱(5μ,250×4.6mm),柱温30℃,流速0.6ml/min,检测波长210nm,进样量15μl,流动相0.1mol/LKH2PO4(用磷酸调至pH2.8)。该方法有机酸添加回收率达到92.5%～101.3%,相对标准差0.62%～2.73%,各种有机酸浓度与HPLC峰面积的线性相关系数大于0.94,表明该测定方法快速简便,具有较高的准确度和精确度。
译  名：
Simultaneous Determination of Various Organic Acids in Compressed Tea by High Performance Liquid Chromatography
作  者：
DING Ling, TU Youying, CHEN Xiaomin (Department of tea science, Zhejiang University, Hangzhou 310029, China)
关键词：
High performance liquid chromatography; determination;organic acid; compressed tea
摘  要：
The determination of ten organic acids in compressed tea by using high performance liquid chromatography was investigated. Organic acids were analysed by using a Shimadzu LC-2010 HPLC equipped with a UV detector and a Diamonsil C18 column,15 μl sample was injected into the column. The detection was performed by measuring absorbance at 210 nm and column temperature at 30℃. The flow rate was set at 0.6 ml/min and mobile phase was 0.1 mol/L KH 2PO 4 (pH value was adjusted to 2.8 by phosphoric acid). The recoveries of the spiked organic acids and the relative standard deviations of the organic acids were 92.5%~101.3%, and 0.62%~2.73%, respectively. All of the correlation coefficients between the concentration of the organic acids and the HPLC response areas were higher than 0.94, indicating that the method was simple, rapid and accurate.

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