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当前位置: 首页 > 文章 > 超声波提取紫苏叶黄酮的工艺研究 安徽农业科学 2009,37 (5) 2046-2048
Position: Home > Articles > Research on Process of Extracting Flavonoids from Perilla Leaf by Ultrasonic Wave Journal of Anhui Agricultural Sciences 2009,37 (5) 2046-2048

超声波提取紫苏叶黄酮的工艺研究

作  者：
胡晓丹;孙爱东;张德权
单  位：
中国农业科学院农产品加工研究所;北京林业大学生物科学与技术学院
关键词：
超声波;紫苏叶;黄酮;工艺
摘  要：
采用单因素试验研究溶剂浓度、提取温度、超声波功率、料液比、提取时间、提取次数对超声波提取紫苏叶黄酮得率的影响。通过正交试验优化超声波提取紫苏叶黄酮的工艺条件。结果表明:随着乙醇浓度的增加,黄酮得率增加,乙醇浓度大于70%时,黄酮得率增加不明显。提取温度为60℃时,黄酮得率最大。随着超声波功率的增大,黄酮得率呈上升趋势。料液比增大,黄酮得率随之增大,但以不超过1∶20(g/ml)为宜。黄酮得率随提取时间的延长而增加,但超过30 min后,增加趋势不明显,各因素对黄酮得率的影响依次为:提取温度>提取时间>料液比>超声波功率。70%乙醇为提取剂时,超声波提取黄酮的最佳工艺为:料液比1∶20(g/ml),提取温度60℃,超声功率150 W,提取次数2次(每次40 min),该条件下紫苏叶黄酮的得率为2.93%,粗提物中黄酮含量为15.46%。
译  名：
Research on Process of Extracting Flavonoids from Perilla Leaf by Ultrasonic Wave
作  者：
HU Xiao-dan et al(College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083)
关键词：
Ultrasonic wave;Perilla leaf;Flavonoids;Process
摘  要：
The single factor test was taken to study the effects of solvent concn.,extraction temperature,ultrasonic power,ratio of material to liquid,extraction time and extraction times on yield rate of flavonoids from perilla leaf by ultrasonic wave.Through the orthogonal test,the process of extracting flavonoids by ultrasonic wave was optimized.The results showed that with the increase of ethanol concn,the yield rate of flavonoids increased,but when ethanol concn was more than 70%,the increase of yield rate of flavonoidswas was not obvious.When the extraction temperature was 60 ℃,the yield rate of flavonoids was the highest.With the increase of ultrasonic power,the yield rate of flavonoids increased.When the ratio of material to liquid increased,the yield rate of flavonoids increased,but the suitable ratio of material to liquid was not more than 1∶20(g/ml).The yield rate of flavonoids was increased with the prolonging of extraction time,but when extraction time was more than 30 min,the increasing trend was not obvious.The effects of each factor on yield rate of flavonoids in order was extraction temperature>extraction time> the ratio of material to liquid >ultrasonic power.When 70% ethanol was extractant,the optimum process of extracting flavonoids by ultrasonic wave was ratio of material to liquid of 1∶20(g/ml),extraction temperature of 60 ℃,ultrasonic power of 150 W,extraction times of 2(each times was 40 min),under this condition the yield rate of flavonoids was 2.93%,and flavonoids content in crude extracts was 15.46%.

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