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当前位置: 首页 > 文章 > 辐照处理对魔芋葡甘聚糖肠道益生作用的影响 食品科学 2018 (11) 83-88
Position: Home > Articles > Effect of Gamma Irradiation on the Prebiotic Functions of Konjac Glucomannan FOOD SCIENCE 2018 (11) 83-88

辐照处理对魔芋葡甘聚糖肠道益生作用的影响

作  者：
李美英;冯观萍;徐振林;孙远明
单  位：
华南农业大学食品学院广东省食品质量安全重点实验室
关键词：
魔芋葡甘聚糖;γ-辐照;益生元;益生元指数;短链脂肪酸
摘  要：
为探究辐照改性技术对魔芋葡甘聚糖(konjac glucomannan,KGM)肠道益生性的影响,以60Co为辐射源制备0、10、20、30 k Gy辐照剂量的KGM样品,采用健康志愿者粪便体外厌氧发酵,比较不同辐照剂量KGM体外发酵液p H值、短链脂肪酸含量、有益菌及有害菌数目、双歧杆菌数量/大肠杆菌数量(B/E值)、益生元指数(prebiotic index,PI)的差异。结果表明:相对于葡萄糖阳性对照,不同辐照剂量KGM均能有效降低发酵液p H值,维持酸性环境,促进短链脂肪酸生成,同时改善发酵液微生物构成,提高B/E值及PI。在0~30 k Gy辐照范围内,随辐照剂量增大,KGM发酵液短链脂肪酸生成量增加,PI升高,0、10、20、30 k Gy KGM的PI分别为0.48、0.61、0.76、0.78。综上,KGM具有良好的肠道益生元作用,γ-辐照改性有助于进一步提高KGM的肠道益生性,综合考虑辐照效率及KGM肠道PI,推荐20 k Gy作为制备KGM低聚糖的适宜辐射剂量。
译  名：
Effect of Gamma Irradiation on the Prebiotic Functions of Konjac Glucomannan
作  者：
LI Meiying;FENG Guanping;XU Zhenlin;SUN Yuanming;Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science,South China Agricultural University;
关键词：
konjac glucomannan;;gamma irradiation;;prebiotic;;prebiotic index;;short chain fatty acid
摘  要：
This work aimed to explore the effect of gamma irradiation on the fermentation characteristics and the prebiotic functions of konjac glucomannan(KGM). KGM was irradiated with 60 Co gamma at doses of 0, 10, 20 and 30 k Gy. The influences of different radiation doses on the changes in p H, short-chain fatty acid(SCFA) content, the numbers of harmful and beneficial bacteria, the ratio between the numbers of bifidobacteria and Escherichia coli(B/E ratio) and prebiotic index(PI) were compared during in vitro anaerobic fermentation of feces from healthy volunteers. Results showed that the p H of the fermentation broth decreased whereas B/E ratio and PI increased significantly in the irradiation groups when compared with the positive control glucose. Meanwhile, gamma irradiation could also contribute to the production of SCFA, as well as the improvement of the microbial composition of the fermentation broth. The contents of SCFA and PI value increased with increasing radiation dose ranging from 0 to 30 k Gy. PI values of KGM irradiated at 0, 10, 20 and 30 k Gy were 0.48, 0.61, 0.76 and 0.78, respectively. Thus, KGM exerts a good prebiotic effect in the gut and its prebiotic functions can be enhanced by gamma irradiation. Taking PI into account, 20 k Gy was recommended as a suitable irradiation dose for KGM.

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