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碱性蛋白酶水解玉米蛋白工艺条件的研究

作  者:
刘振春;董源;王朝辉
单  位:
吉林农业大学食品科学与工程学院
关键词:
玉米蛋白;碱性蛋白酶;水解;水解度
摘  要:
通过单因素分析,对影响碱性蛋白酶水解玉米蛋白的主要因素进行了研究,利用正交试验筛选出最佳工艺参数。结果表明,酶水解最佳条件为底物浓度3%、酶浓度(E/S)3.0%、水解时间1h、温度45℃,在pH值为9.0条件下,水解度可达38.12%。
译  名:
Study on Alkaline Protease Hydrolysis of Corn Gluten Meal
作  者:
LIU Zhen-chun,DONG Yuan,WANG Zhao-hui*(College of Food Engineering,Jilin Agricultural University,Changchun 130118,China)
关键词:
corn gluten meal;alkaline protease;hydrolysis;degree of hydrolysis
摘  要:
The factors affecting hydrolysis of corn gluten meal by alkaline protease were studied by mono-factor analysis,and the optimum technique parameters were determined by orthogonal test.The results showed that under the optimal conditions of:concentration of substrate 3%,enzyme to substrate ratio 3.0%,temperature 45 ℃,hydrolysis time 1 h and pH 9.0,the hydrolysis of degree is 38.12%.

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