当前位置: 首页 > 文章 > 超声波辅助酶法水解玉米蛋白的工艺研究 食品科学 2009,30 (12) 61-65
Position: Home > Articles > Ultrasonic-assisted Enzymatic Hydrolysis of Corn Protein FOOD SCIENCE 2009,30 (12) 61-65

超声波辅助酶法水解玉米蛋白的工艺研究

作  者:
刘振春;邓子瑜;李慧;段绪
单  位:
吉林农业大学食品科学与工程学院
关键词:
玉米蛋白;水解度;超声波
摘  要:
本实验对玉米蛋白在碱性条件下的酶解工艺进行研究,提出一种超声波预处理和碱性蛋白酶酶解相结合的方法,可在短时间内提高产品得率。通过单因素试验及正交试验的考察,超声波辅助酶法水解玉米蛋白的最佳工艺为:超声波预处理时间4min、底物浓度2%、酶浓度5%、pH10.5、温度55℃。在此条件下,玉米蛋白的水解度为61.47%。
译  名:
Ultrasonic-assisted Enzymatic Hydrolysis of Corn Protein
作  者:
LIU Zhen-chun,DENG Zi-yu,LI Hui,DUAN Xu(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)
关键词:
corn protein;hydrolysis degree;ultrasonic
摘  要:
The present study aimed to develop a combination method of ultrasonic pre-treatment and alkaline protease-catalyzed hydrolysis for corn protein.The ultrasonic-assisted enzymatic hydrolysis was optimized through orthogonal test design based on single factor test.When ultrasonic treatment duration,mass fractions of substrate and enzyme,and pH value and temperature for hydrolysis were 4 min,2% and 5%,and 10.5 and 55 ℃,respectively,the maximum hydrolysis degree was achieved,reaching 61.47%.Moreover,molecular weights of products obtained at different hydrolysis time were determined by SDS-PAGE.

相似文章

计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 1

所属期刊

推荐期刊