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Position: Home > Articles > Enzymatic Hydrolysis of Phospholipids for Preparing Lysophospholipids in Organic Phase FOOD SCIENCE 2012,33 (18) 123-126

有机相酶法制备溶血磷脂工艺

作  者:
孙清瑞;张东杰;刘志明;鹿保鑫;王琴;张清荣
单  位:
黑龙江八一农垦大学食品学院
关键词:
溶血磷脂;磷脂;磷脂酶A1;响应面分析法
摘  要:
以大豆浓缩磷脂为原料,通过响应面分析法研究有机相酶法制备溶血磷脂的工艺条件,得出溶血磷脂酸值与影响因素间的回归模型,根据模型进行工艺参数优化。同时,用红外光谱法和高效液相色谱法对所得磷脂进行分析。结果表明,在正己烷有机相中利用磷脂酶A1制备溶血磷脂的最佳工艺参数:反应温度50℃、反应时间13h、加酶量4%、加水量17%、磷脂质量浓度24g/100mL,该条件下磷脂酸值为73.8mg KOH/g。该脱油溶血磷脂中磷脂酰胆碱和1-酰基-溶血磷脂酰胆碱含量分别为6.79%和4.45%。
译  名:
Enzymatic Hydrolysis of Phospholipids for Preparing Lysophospholipids in Organic Phase
作  者:
SUN Qing-rui,ZHANG Dong-jie,LIU Zhi-ming,LU Bao-xin,WANG Qin,ZHANG Qing-rong(College of Food,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
关键词:
lysophospholipids;phospholipids;phospholipase A1;response surface methodology(RSM)
摘  要:
This study deals with the optimization of process conditions for the hydrolysis of phospholipids with phospholipase A1 in hexane for preparing lysophospholipids using response surface methodology.A regression model for acid value of lysophospholipids was established with respect to four hydrolysis parameters.Meanwhile,lysophospholipids were analyzed by infrared spectroscopy and high performance liquid chromatography.The optimal process parameters for preparing lysophospholipids were determined as 50 ℃ of temperature,13 h of reaction time,4% of phospholipase A1,17% of water,and 0.24 g/mL of substrate concentration.The acid value and the contents of phophatidylcholine and 1-acyl-lysophophatidylcholine in the obtained lysophospholipids were 73.8 mg KOH/g,6.79% and 4.45%,respectively.

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