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Position: Home > Articles > Molecular Cloning and Sequence Analysis of the Amino Acid Transporter b~(0,+)AT-2 of Pigs Chinese Journal of Animal and Veterinary Sciences 2009,40 (4) 595-601

猪氨基酸转运载体b~(0,+)AT-2的分子克隆及其序列分析

作  者:
职爱民;冯定远;周响艳;黄志毅;邹仕庚;王修启;左建军;叶慧;王小兰;刘准
单  位:
河南农业科学院河南省动物免疫重点实验室;华南农业大学动物科学学院;东莞泛亚太生物技术有限公司
关键词:
氨基酸转运载体;b0,+AT-2;SLC7A9;克隆;序列分析
摘  要:
本研究克隆了b0,+AT-2的全长cDNA序列。基于NCBI发布的人和鼠b0,+AT序列,应用生物学软件进行序列比对后在同源区设计引物,获取cDNA片段;再利用RACE技术克隆了其全长cDNA序列。生物信息学分析显示:猪b0,+AT-2的全长1 488 bp,包括90 bp的3′非翻译区(UTR)和126 bp的5′UTR;编码区(CDS)编码423个氨基酸残基,分别和人、鼠b0,+AT以及猪b0,+AT-1有75.1%、71.9%和86.6%的同源性。
译  名:
Molecular Cloning and Sequence Analysis of the Amino Acid Transporter b~(0,+)AT-2 of Pigs
作  者:
ZHI Ai-min1,2,FENG Ding-yuan1,ZHOU Xiang-yan3,HUANG Zhi-yi1,ZOU Shi-geng1,WANG Xiu-qi1,ZUO Jian-jun1,YE Hui1,WANG Xiao-lan1,LIU Zhun1 (1.College of Animal Science,South China Agricultural University,Guangzhou 510642,China;2.Henan Provincial Key Laboratory for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450001,China;3.ASIAPAC(Dongguan) Biotechnology Co,Ltd,Dongguan 523808,China)
关键词:
Amino Acid Transporter;b0,+AT-2;SLC7A9;cloning;sequence analysis
摘  要:
The cloning,sequencing of porcine b0,+AT transcript variant 2 was described in this study.Based on the sequences of human and mouse b0,+AT published on the NCBI,we identified a partial cDNA using the primers designed after bioinformatics analysis.Using rapid amplification of cDNA ends(RACE),the full-length cDNA encoding porcine b0,+AT-2 was sequenced.The porcine b0,+AT-2 cDNA was 1 488 bp long,including 90 bp 3′UTR and 126 bp 5′UTR,and CDS encoded 423 amino acids.The b0,+AT-2 protein sequence was found to be 75.1%,71.9% and 86.6% identities with human b0,+AT,mouse b0,+AT and swine b0,+AT-1.

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