作 者：
朱晓彤;张方亮;尹杰;黎育颖;韩慧;宾石玉;李铁军;印遇龙
关键词：
β-甘露聚糖酶;半乳甘露聚糖;豆粕;氨基酸转运载体;生化指标;断奶仔猪
摘 要：
本试验在分析了22种饲料原料中α-半乳甘露聚糖(α-GM)、β-半乳甘露聚糖(β-GM)和半乳甘露聚糖(GM)含量的基础上,研究了饲粮豆粕水平与β-甘露聚糖酶(β-MN)对断奶仔猪血清α-GM、β-GM、GM含量,血清生化指标,以及肠道溶质载体家族7成员1(SLC7A1)、溶质载体家族7成员11(SLC7A11)、溶质载体家族38成员2(SLC38A2)基因相对表达量的影响。试验采用2×2因子设计,将24头初始体重接近的健康二元杂交断奶仔猪随机分为4组(Ⅰ、Ⅱ、Ⅲ、Ⅳ组),每组6个重复,每个重复1头猪。Ⅰ组饲喂22%豆粕水平饲粮,Ⅱ组饲喂22%豆粕水平并添加0.02%β-MN的饲粮,Ⅲ组饲喂37%豆粕水平饲粮,Ⅳ组饲喂37%豆粕水平并添加0.02%β-MN的饲粮。试验期为30 d。在第30天对24头仔猪进行颈动脉、肠系膜静脉以及肝门静脉采血10 m L,检测血清中α-GM、β-GM和GM含量;对前腔静脉采血10 m L,检测血清生化指标;并取空肠前端和回肠后端,检测SLC7A1、SLC7A11、SLC38A2基因相对表达量。结果表明:与Ⅰ组相比,Ⅲ组断奶仔猪平均日采食量(ADFI)和回肠SLC7A1基因相对表达量均显著降低(P<0.05),Ⅲ组断奶仔猪平均日采食α-GM含量,颈动脉血清β-GM含量,肠系膜静脉血清β-GM、GM含量,前腔静脉血清谷丙转氨酶、谷草转氨酶活性和尿素氮含量均显著升高(P<0.05);Ⅱ组断奶仔猪肠系膜静脉血清α-GM含量及肝门静脉血清α-GM和GM含量均显著降低(P<0.05),前腔静脉血清葡萄糖、钙和高密度脂蛋白含量均显著升高(P<0.05)。与Ⅲ组相比,Ⅳ组断奶仔猪肝门静脉血清GM含量显著降低(P<0.05),空肠SLC7A1、SLC7A11和SLC38A2基因相对表达量均显著升高(P<0.05)。由此可见,仔猪平均日采食α-GM的含量升高,可降低仔猪ADFI;肝门静脉、颈动脉和肠系膜静脉血清α-GM、β-GM和GM含量随饲粮α-GM、β-GM和GM含量的增加而增加。饲粮α-GM、β-GM和GM含量增加时,添加β-MN可降低肝门静脉血清GM含量,上调空肠SLC7A1、SLC7A11和SLC38A2基因相对表达量。
译 名：
Effects of Dietary Soybean Meal Level and β-Mannanase on Serum Galactomannan Content,Biochemical Indices and Intestinal Amino Acid Transporters Gene Expression of Weaner Piglets
作 者：
ZHU Xiaotong;ZHANG Fangliang;YIN Jie;LI Yuying;HAN Hui;BIN Shiyu;LI Tiejun;YIN Yulong;College of Life Science,Guangxi Normal University;Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central,Ministry of Agriculture;University of Chinese Academy of Sciences;Hunan Co-Innovation Center of Animal Production Safety;
关键词：
β-mannanase;;galactomannan;;soybean meal;;amino acid transporter;;biochemical indices;;weaner piglets
摘 要：
According to the analysis of α-galactomannan( α-GM),β-galactomannan( β-GM) and galactomannan( GM) contents in 22 kinds of feed ingredients,this study evaluated the effect of β-mannanase( β-MN) on the contents of α-GM,β-GMand GMin serum,serum biochemical indices,and the gene relative expression of solute carrier family 7 member 1( SLC7 A1),solute carrier family 7 member 11( SLC7 A11)and solute carrier family 38 member 2( SLC38 A2) of weaner piglets. Twenty-four healthy dualistic hybrid weaner piglets with similar body weight were randomly divided into 4 groups( groups Ⅰ,Ⅱ,Ⅲ and Ⅳ) with6 replicates per group and 1 pig per replicate in a 2 × 2 factorial design. Piglets in group Ⅰ fed the 22% soybean meal( SBM) level diet,piglets in group Ⅱ fed the 22% SBMlevel diet supplemented with 0. 02% β-MN,piglets in group Ⅲ fed the 37% SBMlevel diet,and the others in group Ⅳ fed the 37% SBMlevel diet supplemented with 0.02% β-MN. The experiment lasted for 30 days. At day 30,10 m L blood samples from carotid artery,mesenteric vein and portal vein were collected from 24 piglets for serum α-GM,β-GMand GMcontents determination,respectively; 10 m L blood from precaval vein used for serum biochemical indices analysis.The proximal jejunum and distal ileum samples were collected for analysis gene relative expression of SLC7 A1,SLC7 A 11 and SLC38 A 2. The results showed that compared with group Ⅰ,the average daily feed intake( ADFI) and SLC7 A1 gene relative expression in ileum of weaner piglets in group Ⅲ were significantly decreased( P<0.05),the average daily intake of α-GMcontent,the content of β-GMin carotid artery serum,the contents of β-GMand GMin mesenteric vein serum,the alanine aminotransferase,glutamic oxaloacetic transaminaseand activities and urea nitrogen content in precaval vein serum of weaner piglets in group Ⅲ were significantly increased( P< 0.05); the content of α-GMin mesenteric vein serum,the contents of α-GMand GMin portal vein serum of weaner piglets in group Ⅱ were significantly decreased( P < 0. 05),the contents of glucose,calcium and high density lipoprotein in precaval vein serum were significantly increased( P < 0. 05).Compared with Ⅲ group,the content of GMin portal vein serum of weaner piglets in group Ⅳ was significantly decreased( P< 0. 05),the gene relative expressions of SLC7 A1,SLC7 A11 and SLC38 A2 in jejunum were significantly increased( P< 0. 05). These findings suggest that with the average daily intake of α-GMcontent increase,the ADFI is decreased; the contents of α-GM,β-GM,and GMin carotid artery,mesenteric vein,and hepatic portal vein serum are increased by the dietary α-GM,β-GMand GMcontents increase. W hen dietary α-GM,β-GMand GMincrease,β-MN can decrease the content of GMin portal vein serum and increase the gene relative expression of SLC7 A1,SLC7 A11 and SLC38 A2 in jejunum.
