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当前位置: 首页 > 文章 > Dynamic Expression of miRNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings 园艺学报 2016,2 (1) 41-49
Position: Home > Articles > Dynamic Expression of miRNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings Acta Horticulturae Sinica 2016,2 (1) 41-49

Dynamic Expression of miRNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings

作  者：
Chaohan Li;Yansu Li;Longqiang Bai;Chunguang He;Xianchang Y
单  位：
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, Chin; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词：
cucumber;graft;drought;miRNA;targe
摘  要：
Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. MicroRNAs (miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber (Cucumis sativus) scions onto pumpkin (Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated grafting-induced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target mRNAs were detected by quantitative real-time PCR (qRT-PCR) in leaves and roots of hetero-grafted cucumber seedlings (cucumber as scion and pumpkin as rootstock) and auto-grafted cucumber seedlings (cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3?h of drought stress, and then induced after 8?h and 24?h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1?h, but induced after 3?h and 8?h, then reduced significantly after 24?h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance.
单  位：
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China%Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China%Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China%Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
关键词：
cucumber%graft%drought%miRNA%target
摘  要：
Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. MicroRNAs (miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber (Cucumis sativus) scions onto pumpkin (Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated grafting-induced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target mRNAs were detected by quantitative real-time PCR (qRT-PCR) in leaves and roots of hetero-grafted cucumber seedlings (cucumber as scion and pumpkin as rootstock) and auto-grafted cucumber seedlings (cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3?h of drought stress, and then induced after 8?h and 24?h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1?h, but induced after 3?h and 8?h, then reduced significantly after 24?h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance.

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