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当前位置: 首页 > 文章 > 福建家养淡水鱼暴发传染病的细菌分离鉴定与防治 福建农业学报 2004,19 (4) 23-28
Position: Home > Articles > Isolation and identification of the bacteria causing acute epidemic septicemia in cultured fresh water fishes and the treatment Fujian Journal of Agricultural Sciences 2004,19 (4) 23-28

福建家养淡水鱼暴发传染病的细菌分离鉴定与防治

作  者：
孔繁德;陈琼;王生育;黄印尧;陈信忠;龚艳清
单  位：
厦门出入境检验检疫局;厦门出入境检验检疫局 福建 厦门361012;厦门市农产品质量安全检验测试中心;厦门格灵生物技术有限公司
关键词：
鱼;迟缓爱德华氏菌;弗氏枸掾酸杆菌;分离鉴定;防治
摘  要：
2003年下半年福建省龙海市许多养鱼场发生一起以出血为主的家养鱼暴发性传染病，对其进行了病原分离鉴定。通过常规细菌学鉴定和应用 ATB 全自动鉴定系统，从濒死的鲢鱼和罗非鱼的实质性脏器中各分离出1株迟缓爱德华氏菌(Edwardstella tarda)，同时还从鲢鱼中分离出1株弗氏枸掾酸杆菌(Citrobacter freandn)。用这些菌人工感染小鼠和健康鱼，表明这些菌对小鼠有极强的毒力，对鱼表现出与自然发病鱼类似的病症，并回收到与接种菌一致的细菌。选择敏感性高的丙沙星、氟哌酸、菌必治交叉使用．同时搞好周围环境卫生，有效地控制了病情。
译  名：
Isolation and identification of the bacteria causing acute epidemic septicemia in cultured fresh water fishes and the treatment
作  者：
KONG Fan-de~1 CHEN Qiong~2 WANG Sheng-yu~3 HUANG Ym-yao~1 CHEN Xin-zhong~1 GONG Yan-qing~11.Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen,Fujian 361012.China;2.Xiamen Agricultural Product Quality and Safety Testing Centre,Xiamen.Fujian 361009,China;3.Xiamen Gelin Biological and Technological Ltd.Co.,Xiamen,Fujian 361004,China
关键词：
Fish;;Edwardsiella tarda;;Citrobacter freundii;;Isolation and Identification;;Treatment
摘  要：
Systemic investigations on the pathogen of epidemic septicemia,a new infectious disease in fishes(mainlysilver carps,tilapia)which occurred among cultured fishes in South China in summer to early-autumn of 2003 werecarried out.Two strains of Edwardstella tarda(Et)and a strain of Citrobacter freundn(Cf)were isolated fromkidney of moribund fishes with hemorrhage in dermis,vent,fin,gill and internal organs.Four-tube method andclinic symptom indicated that two isolates belong to Et and a isolate Cf.In addition.VITEK-JR30 system wasused to identify these isolates and reconfirmed the results.Pathogenic test documented that the bacteria are thecausative agents of the disease.After experimentally infected by the bacteria via intraperitoneal inoculation,fishesdied of hemorrhagic scpticemia,similar to naturally infected fish.The piscine diseases were effectively controlledby using sensitive drugs which had been proven to be strongly susceptive to these strains.

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