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Position: Home > Articles > STUDIES ON STRUCTURAL POLYPEPTIDES OF RABBIT HAEMORRHAGIC DISEASE VIRUS(RHDV) Acta Agriculturae Shanghai 1999 (4) 61-64

兔出血症病毒结构多肽的研究

作  者:
王恒安;杜念兴;徐为燕
单  位:
南京农业大学;上海农学院动物科学系
关键词:
兔出血症病毒;结构多肽;免疫转印
摘  要:
纯化RHDV 经SDSPAGE、硝酸银染色显示8 条多肽,分子量分别为85.1,71.2,64.5,61.4,57.3,54.0,29.2,28.1 kD,免疫转印结果确认其中的6 条多肽为病毒结构多肽,分子量分别为64.5,61.4,57.3,54.0,29.2,28.1 kD,且其相对百分含量分别为65.87,3.17,3.97,10.32,9.52,7.14% ,表明分子量为64.5 kD的多肽为病毒主要结构多肽。
译  名:
STUDIES ON STRUCTURAL POLYPEPTIDES OF RABBIT HAEMORRHAGIC DISEASE VIRUS(RHDV)
作  者:
Wang Hengan1) Du Nianxing2) Xu Weiyan2) (1)Departm entof Anim alScience,ShanghaiAgriculturalCollege,Shanghai201101; 2)Nanjing AgriculturalUniversity,Nanjing 210095)
关键词:
Rabbithaem orrhagic disease virus;Structuralpolypeptides;Im m unoblotting
摘  要:
Eight polypeptides of the purified RHDV,w ith m olecular w eight of 85.1, 71.2,64.5,61.4,57.3,54.0,29.2 and 28.1 kD,w ere detected by SDS PAGE stained w ith silver. By im m unoblotting analysis, six of them w ere determ ined to be viral structural polypeptides w ith theirrespective m olecularw eightsof64.5,61.4,57.3,54.0,29.2 and 28.1 kD,and the relative quantities of the six polypeptides w ere 65.87% , 3. 17% , 3. 97% , 10.32% ,9.52% and 7.14% ,respectively.This resultshow ed thatthe polypeptide of64.5 kD w as the m ain structuralpolypeptide ofRHDV.

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