作 者：
韩亚楠;杨晨玉;王子旭;陈耀星;孙佳毅;秦卓明;曹静;董玉兰
单 位：
中国农业大学生物学院;山东省农业科学院家禽所;中国农业大学动物医学院
摘 要：
旨在探究机体中5-HT水平与应激腹泻导致的肠道氧化应激的相关性,进一步揭示5-HT在胃肠道功能中的作用。将40只21日龄断奶仔鼠分成对照组、应激腹泻组、PCPA注射对照组(阻断5-HT合成)和PCPA注射腹泻组,利用ELISA和生物化学的方法检测5-HT和氧化产物、抗氧化酶的含量。结果表明:(1)仔鼠断奶应激腹泻导致血浆5-HT含量显著升高,比对照组升高23.42%(P=0.009);PCPA腹腔注射对正常小鼠血浆5-HT含量影响不显著(P=0.200),但是能够有效阻断应激腹泻时5-HT的合成(P=0.006);(2)与对照组相比,应激腹泻时不同肠段组织氧化产物(MDA)水平均显著增加,尤其十二指肠及空肠增加明显,分别升高了2.70倍(P=0.000)、3.06倍(P=0.000);各肠段GSH-PX、CAT、SOD及T-AOC抗氧化酶水平均出现不同程度降低,十二指肠GSHPX降低了70.59%(P=0.000),SOD活性降低了59.15%(P=0.001),T-AOC下降53.01%(P=0.000);空肠SOD活性下降明显,降低71.05%(P=0.000);回肠GSH-PX、CAT、SOD水平分别下降72.33%(P=0.000)、50.82%(P=0.000)、73.86%(P=0.000);结肠CAT水平降低显著,由正常水平(0.85±0.06)U·mg-1降低至(0.46±0.09)U·mg-1,下降72.60%(P=0.000);(3)5-HT合成阻断后,应激腹泻组不同肠段组织抗氧化功能均有不同程度恢复,但与正常组相比仍差异显著。结果提示应激腹泻造成肠道抗氧化功能严重损伤,而阻断5-HT合成能有效改善其抗氧化功能,从而减轻腹泻病理程度。
译 名：
Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice
作 者：
HAN Ya-nan;YANG Chen-yu;WANG Zi-xu;CHEN Yao-xing;SUN Jia-yi;QIN Zhuo-ming;CAO Jing;DONG Yu-lan;College of Veterinary Medicine,China Agricultural University;College of Biology,China Agricultural University;Department of Biological Sciences Purdue University,West Lafayette,IN47907,USA;Poultry institute,Shandong Academy of Agricultural Science;
关键词：
serotonin;;weaning stress-diarrhea;;intestine;;antioxidant function
摘 要：
This study aimed to explore the relationship between 5-HT level and antioxidant function in the weaning stress-diarrhea intestine and further revealed the role of 5-HT in the gastrointestinal tract function.Forty 21-day-old weaned mice were divided into four treatment groups:Control,PCPA Control(block the synthesis of 5-HT),Stress-diarrhea and PCPA+ stress-diarrhea.Plasma 5-HT was determined using ELISA kit.Intestinal oxidation products and antioxidant enzyme activity were determined by spectrophotometry.The results showed:(1)Weaning stressdiarrhea significantly increased plasma 5-HT concentration by 23.42%(P=0.009)comparedwith control group.Pretreated with PCPA decreased,plasma 5-HT concentration which had no significant difference in normal mice(P=0.200),yet totally prevented the increase in 5-HT levels in weaning stress-diarrheal mice(P=0.006).(2)Oxidative product(MDA)level was significantly increased in intestine of stress-diarrhea group,and it increased by 2.70 time and 3.06 time than that of control group especially in duodenum and jejunum(P=0.000);GSH-PX,CAT,SOD and T-AOC activities appeared decrease in a different extent in intestine of stress-diarrhea group.GSH-PX,SOD and T-AOC activities in duodenum were respectively reduced by 70.59%(P=0.000),59.15%(P=0.001)and 53.01%(P=0.000).SOD activity in jejunum was significantly reduced by 71.05%(P=0.000)compared with that of control group.GSH-PX,CAT and SOD activities in ileum were respectively reduced by 72.33%(P=0.000),50.82%(P=0.000),73.86%(P=0.000).CAT activity in colon decreased significantly to 0.46±0.09U·mg-1(from0.85±0.06 U·mg-1).(3)Pretreated with PCPA in the weaning stress-diarrheal mice could promote the intestinal antioxidant function,but this did not bring the mice to recovery.The results suggested that weaning stress-diarrhea could cause serious injury of intestinal antioxidant function,while blocking the synthesis of 5-HT could effectively improve its antioxidant function and relieve diarrhea symptom.
