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Position: Home > Articles > Isolation and Idenfication of Cellulase-producing Bacuillus Strains in Tibetan Sheep Rumen Progress in Veterinary Medicine 2016,37 (4) 70-73

藏绵羊瘤胃中产纤维素酶芽胞杆菌的分离鉴定

作  者:
彭忠利;邹智坤;曾泽;张斌;岳华;冯浩媛;汤承
单  位:
西南民族大学生命科学与技术学院
关键词:
藏绵羊;瘤胃内容物;产纤维素酶芽胞杆菌;分离鉴定
摘  要:
2013年10月,采集10份四川阿坝藏族羌族自治州若尔盖藏绵羊瘤胃内容物。采用高温水浴,富集培养的方法进行芽胞杆菌的分离,并使用刚果红染色法进行产纤维素酶芽胞杆菌的初步筛选,共获得101株产纤维素酶芽胞杆菌。通过16SrRNA序列测定对分离出的芽胞杆菌鉴定出3种不同的芽胞杆菌,其中短小芽胞杆菌78株、蜡样芽胞杆菌14株、枯草芽胞杆菌9株。本研究鉴定了藏绵羊瘤胃中芽胞杆菌的组成,为进一步利用芽胞杆菌开发藏绵羊源的益生菌奠定了基础。
译  名:
Isolation and Idenfication of Cellulase-producing Bacuillus Strains in Tibetan Sheep Rumen
作  者:
PENG Zhong-li;ZOU Zhi-kun;ZENG Ze;ZHANG Bin;YUE Hua;FENG Hao-yuan;TANG Cheng;College of Life Science and Technology,Southwest University for Nationalities;
关键词:
Tibetan sheep;;rumen content;;cellulase producing Bacillus;;isolation and identification
摘  要:
In october 2013,10 Tibetan sheep rumen content samples were collected in a slaughter house of Ruoergai County,Aba Tibetan and Qiang Autonomous Prefecture,Sichuan.Bacillus were isolated with the high temperature water bath and the method of enrichment culture.The preliminary screening of cellulase producing Bacillus were conducted by using Congo red staining.101 strains of cellulase producing Bacillus were obtained.Through the 16 SrRNA sequence determination,3 species of Bacillus were identified,including,78 strains of Bacillus pumilus,14 strains of Bacilluscereus,9 strains of Bacillus subtilis.In this study,the compositions of Bacillus in Tibetan sheep rumen were identified for further utilization of Bacillus from Tibetan sheep to develop the probiotics.

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