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Position: Home > Articles > Study on hepatic cells apoptosis induced by olaquindox in Cyprinus carpio Journal of Fisheries of China 2004,28 (6) 126-130

喹乙醇诱导鲤肝细胞凋亡的研究

作  者：
汪开毓;赵德明;耿毅;黄小丽;刘光迅
单  位：
四川省水产研究所;四川农业大学水产系;中国农业大学动物医学院
关键词：
鲤;细胞凋亡;喹乙醇;肝细胞
摘  要：
Hepatic cells apoptosis induced by olaquindox in common carp, Cyprinus carpio, were studied. The test was conducted for 30 days, with dose of 200mg·kg~(-1) feed. Terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling (TUNEL), electronic microscopic and flow cytometry were performed to analyze apoptosis. The result showed that hepatic cells apoptosis were indentified under light microscope with TUNEL staining; chromatin condensation, chromosal clumping and margination, and apoptotic bodies were observed under electronic microscope; and sub-G_1 PEAK (AP peak) in hepatic cells could be detected by flow cytometric method. The apoptotic rate measured by flow cytomtry increased with dye-poison time, on the 7th,14th,20th,25th and 30th day, the apoptotic rate was 2.05%, 6.25%, 9.49%, 13.31% and 17.44% respectively.
译  名：
Study on hepatic cells apoptosis induced by olaquindox in Cyprinus carpio
作  者：
WANG Kai-yu~1, ZHAO De-ming~2, GENG Yi~1, HUANG Xiao-li~1, LIU Guang-xun~3 (1. Department of Fisheries,Sichuan Agricultural University,Yaan625014,China; 2. Veterinary Medicine College, China Agricultural University, Beijing100094,China; 3. Sichuan Fisheries Research Institute, Chengdu611731, China)
关键词：
Cyprinus carpio; apoptosis; olaquindox; hepatic cell
摘  要：
Hepatic cells apoptosis induced by olaquindox in common carp, Cyprinus carpio, were studied. The test was conducted for 30 days, with dose of 200mg·kg~(-1) feed. Terminal deoxynucleotidy transferase mediated dUTP-Biotin nick-end labeling (TUNEL), electronic microscopic and flow cytometry were performed to analyze apoptosis. The result showed that hepatic cells apoptosis were indentified under light microscope with TUNEL staining; chromatin condensation, chromosal clumping and margination, and apoptotic bodies were observed under electronic microscope; and sub-G_1 PEAK (AP peak) in hepatic cells could be detected by flow cytometric method. The apoptotic rate measured by flow cytomtry increased with dye-poison time, on the 7th,14th,20th,25th and 30th day, the apoptotic rate was 2.05%, 6.25%, 9.49%, 13.31% and 17.44% respectively.

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