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当前位置: 首页 > 文章 > 鼠曲草提取物及组分对α–淀粉酶的抑制作用 湖南农业大学学报(自然科学版) 2015,41 (4) 412-415
Position: Home > Articles > Inhibition effects of extract and its compositions from Gnaphlium affine D.Don on α-amylase Journal of Hunan Agricultural University(Natural Sciences) 2015,41 (4) 412-415

鼠曲草提取物及组分对α–淀粉酶的抑制作用

作  者：
陆英;邓林燕;朱丽娜;李觅路
单  位：
湖南农业大学园艺园林学院;国家植物功能成分利用工程技术研究中心
关键词：
鼠曲草;提取物;α–淀粉酶;抑制作用
摘  要：
采用分析型高效液相色谱仪对鼠曲草提取物进行分离、鉴定,并测定了鼠曲草提取物及其中3个组分对α–淀粉酶活性的影响。结果表明:从鼠曲草提取物中分离出7个组分,并鉴定出3个组分,分别为3,5–O–咖啡酰基奎宁酸,3,4–O–咖啡酰基奎宁酸,2′,4,4′–三羟基–6′–甲氧基–查尔酮–4′–O–β–D–葡萄糖;当α–淀粉酶质量浓度达到1.67 mg/m L时,鼠曲草提取物、3,5–O–咖啡酰基奎宁酸、3,4–O–咖啡酰基奎宁酸、2′,4,4′–三羟基–6′–甲氧基–查尔酮–4′–O–β–D–葡萄糖的抑制率分别为32.37%、84.53%、63.07%、71.22%,半抑制浓度IC50分别为2.71、0.90、1.28、1.16 mg/m L;对2′,4,4′–三羟基–6′–甲氧基–查尔酮–4′–O–β–D–葡萄糖进行动力学分析的结果显示,2′,4,4′–三羟基–6′–甲氧基–查尔酮–4′–O–β–D–葡萄糖为可逆非竞争性抑制类型。
译  名：
Inhibition effects of extract and its compositions from Gnaphlium affine D.Don on α-amylase
作  者：
Lu Ying;Den Linyan;Zhu Lina;Li Milu;College of Horticulture and Landscape, Hunan Agricultural University;National research Center of Engineering Technology For Utilization of Functional Ingredients From Botanicals;
关键词：
Gnaphlium affine D.Don;;extract;;α-amylase;;inhibition
摘  要：
Isolation and identification of composition from Gnaphlium affine D.Don extract were conducted with high performance liquid chromatography. Inhibition effects of extract and its three compositions on α– amylase activity were determined. The results showed that seven compositions were isolated from the extract and three of them were identified as 3,5–di–O–caffeoylquinic acid, 3,4–di–O–caffeoylquinic acid, 2′,4,4′–trihydroxy –6′–methoxychalcone–4′–O–β–D– glucopyranoside. The inhibition rates of extract, 3,5–di–O–caffeoylquinic acid, 3,4–di–O–caffeoylquinic acid, and 2′,4,4′–trihydroxy –6′–methoxychalcone–4′–O–β–D–glucopyranoside were 32.37%, 84.53%, 63.07% and 71.22% respectively when the concentration of α– amylase was 1.67 mg/m L, and half inhibitory concentrations(IC50) were 2.71, 0.90, 1.28 and 1.16 mg/m L. The kinetic analysis showed that the inhibition of 2′,4,4′– trihydroxy –6′–methoxychalcone –4′–O–β–D–glucopyranoside on α–amylase was reversible and non–competitive.

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