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Position: Home > Articles > Optimization of Hydrolysis Process for Corn Gluten Meal by Response Surface Methodology FOOD SCIENCE 2012,33 (4) 71-76

响应面法优化玉米黄粉蛋白的酶解工艺

作  者:
郑明洋;王元秀;张桂香;孙纳新
单  位:
山东省医学科学院医学与生命科学学院;山东省医学科学院;济南大学
关键词:
蛋白酶;玉米黄粉蛋白;响应面;酶解工艺
摘  要:
利用pH-stat法测定碱性蛋白酶和中性蛋白酶对玉米黄粉蛋白的水解度,通过Box-Benhnken响应曲面法优化水解条件。根据单因素试验结果设计中心组合试验,以水解度为指标,采用响应面分析法确定最优水解工艺参数。结果表明:蛋白酶水解的最适条件为酶解pH11.10、酶解温度55.00℃、底物质量浓度112g/L、碱性蛋白酶与中性蛋白酶酶活单位比值5:1、加酶量48000U/g、酶解时间120min;在此条件下,玉米黄粉蛋白水解度实测值为30.23%,模型的预期值为30.84%。采用复合酶水解可提高玉米黄粉蛋白水解度,且工艺简单。
译  名:
Optimization of Hydrolysis Process for Corn Gluten Meal by Response Surface Methodology
作  者:
ZHENG Ming-yang1,2,WANG Yuan-xiu2,,ZHANG Gui-xiang2,SUN Na-xin2(1.Shandong Academy of Medical Sciences,Jinan 250062,China; 2.College of Medicine and Life Sciences,Shandong Academy of Medical Sciences-University of Jinan,Jinan 250022,China)
关键词:
protease;corn gluten meal;response surface methodology;hydrolysis
摘  要:
Response surface methodology based on Box-Benhnken experimental design was employed to optimize conditions for the dual-enzymatic hydrolysis of corn gluten meal to obtain the maximum degree of hydrolysis.The optimal hydrolysis conditions were found as follows: pH 11.10,hydrolysis temperature 55.00 ℃,substrate concentration 112 g/L,activity ratio of alkaline protease to neutral protease 5:1,total enzyme dose 48000 U/g,and hydrolysis time 120 min.Under these conditions,the actual degree of hydrolysis was 30.23%,and the predicted value 30.84%.The dual-enzymatic hydrolysis process was simple and provided a high degree of hydrolysis.

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