关键词：
马桑香菇;液体菌种;发酵培养;碳源;氮源;配方优化
摘 要：
采用单因素试验和正交试验方法,从温度、酸碱度、矿物质、碳源、氮源等方面对马桑香菇液体菌种发酵培养的适宜条件进行了研究.结果显示:马桑香菇液体菌种发酵培养的最佳温度为23~25℃,适宜的pH值为6.0~7.0,适宜的矿物质是CaCl2和K2HPO4,适宜的碳源有乳糖、葡萄糖、果糖,适宜的氮源有豆粕粉、麦麸、玉米浆.马桑香菇液体菌种发酵培养的最优培养基配方为:葡萄糖30.0 g,乳糖5.0 g,豆粕粉1.0 g,玉米浆4.0 g,CaCl20.5 g,K2HPO40.5 g,维生素B110 mg,去离子水1000 mL(pH值为6.0).采用最优配方进行验证培养试验,马桑香菇的菌丝干重达0.88 g/100 mL,TTC-脱氢酶最大活力达292.15μg/(mg·h).
译 名：
Research on Fermentation Conditions for Liquid Strain of Lentinula edodes
作 者：
XIONG Xue;WAN Li-ying;XIANG Zhun;LI Peng;HUANG Jing;HE Yao-wei;Guizhou Institute of Biology;Guizhou Edible Fungus Industry System;Liupanshui Vocational and Technical College;
关键词：
Lentinula edodes;;Liquid strain;;Fermentation culture;;Carbon source;;Nitrogen source;;Formula optimization
摘 要：
The optimal conditions for liquid fermentation culture of Lentinula edodes were studied by single-factor and orthogona-Valuel test from the aspects of temperature, pH-value, mineral elements, carbon sources and nitrogen sources in the paper. The results showed that the optimal growth temperature for liquid fermentation of Lentinula edodes was 23～25 ℃.Its suitable pH-value was 6.0～7.0. Its suitable minerals were CaCl_2 and K_2HPO_4. Its suitable carbon sources included lactose,glucose and fructose, and suitable nitrogen sources included soybean powder, wheat bran and corn steep liquor. The optimal fermentation culture formula for liquid strain of Lentinula edodes was the glucose 30.0 g, lactose 5.0 g, soybean powder 1.0 g,corn steep liquor 4.0 g, CaCl_2 0.5 g, K_2HPO_4 0.5 g, vitamin B_1 10 mg. pH-value 6.0 and deionized water 1000 mL. With this formula, the dry weight of Lentinula edodes mycelia reached 0.88 g/100 mL, and the maximum TTC dehydrogenase activity reached 292.15 μg/(mg·h).
