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维生素B_1、B_2含量测定方法比较

作  者:
郭亚东;马银海;李燕华;李璠
单  位:
昆明师范高等专科学校;昆明医学院药学院
关键词:
紫外分光光度法;维生素B1;维生素B2;复合维生素B片
摘  要:
目的:比较维生素B1、B2含量测定方法。方法:采用紫外分光光度法中三种不同方法测定维生素B1、B2含量。结果:1、按E值测标示量维生素B1:98.3±2.51,维生素B2:94.8±0.308,复合维生素B片中维生素B1:102.1±1.70,维生素B1平均回收率为100.4%,RSD为2.13%(n=6),维生素B2平均回收率为94.9%,RSD为6.5%(n=6)。2、标准曲线法维生素B1、B2浓度分别在3~18、1~8μg/ml范围内,均呈良好线性关系,标示量分别为:102.9±0.661,98.4±0.152。3、双波长法测维生素B1标示量:94.2±1.97。结论:三种方法用于维生素B1、B2含量测定,操作简便、快速、准确,精密度好。
译  名:
Comparison in Determination Methods of Vitamin B_1,B_2
作  者:
GUO Ya-dong1,MA Yin-hai2,LI Yan-hua1,LI Fan1(1.Kunming Medical College,Kunming 650031,China;2.Kunming Normal College,Kunming 650031,China)
关键词:
UV;vitamin B1;vitamin B2;compound vitamin B tablets
摘  要:
Objective: To compare the determination methods of VB1, VB2. Methods: the three different methods of UV fordetermining VB1, VB2 are adopted. Results: According to the value of E, the contents were 98.3±2.51 for VB1 and 94.8±0.308 for VB2, the VB1 content of compound vitamin B tablets was 102.1±1.70. The average recovery for VB1 and VB2 were100.4 and 94.9 respectively. According calibration curve method: the concentration of VB1 and VB2 were 3~18 and 1~8μg/ml, showed good linear relationship. The results were 102.9±0.661 for VB1 and 98.4±0.152 for VB2. The dual wavelengthspectrophotometery: the content of VB1 was 04.8±1.97. Conclutions: The three methods could be used to the determination of VB1,VB2.They are simple, rapid, accurate, the precision are very good.

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