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当前位置: 首页 > 文章 > 转Bt基因油菜的抗虫性分析 湖北农业科学 2016 (9) 2386-2391+2422
Position: Home > Articles > Insect Resistance Analysis on the Transgenic Bt Gene Brassica napus L. Hubei Agricultural Sciences 2016 (9) 2386-2391+2422

转Bt基因油菜的抗虫性分析

作  者：
万丽丽;王转茸;辛强;董发明;洪登峰;杨光圣
单  位：
华中农业大学作物遗传改良国家重点实验室
关键词：
油菜(Brassica napus L.);苏云金芽孢杆菌;Cry1C;Cry2A;双价聚合;农杆菌介导遗传转化;品质性状
摘  要：
利用农杆菌介导的遗传转化分别将Cry1C和Cry2A的2个单价Bt基因转入油菜(Brassica napus L.),以两个纯合的转基因抗虫油菜为亲本,通过有性杂交的方法将不同Bt基因聚合,培育双价Bt抗虫油菜,并对其抗虫性和种子品质性状进行评价。结果表明,Cry1C、Cry2A在聚合后均能稳定表达,和单价Cry1C转基因植株相比,双价株系中蛋白质含量明显降低,以Cry2A为母本的聚合株系蛋白质含量降低更多,Cry1C在遗传上存在母本效应。室内接种小菜蛾二龄幼虫结果显示,转化单价和双价聚合Bt基因的抗虫性增强,其中转化单价Cry1C的抗虫性优于双价聚合Bt基因和单价Cry2A基因的转基因植株。玻璃温室栽培转基因植株,单价Bt和双价聚合Bt基因的转基因植株能生存而非转基因植株受到严重虫害而死亡。对抗性优良的单价和双价聚合的转基因植株种子品质测定发现,与未受到虫害的受体材料双低优良恢复系7-5的含油量和硫苷含量差异不显著,从而达到抗性改良的目的。
译  名：
Insect Resistance Analysis on the Transgenic Bt Gene Brassica napus L.
作  者：
WAN Li-li;WANG Zhuang-rong;XIN Qiang;DONG Fa-ming;HONG Deng-feng;YANG Guang-sheng;National Key laboratory of Crop Genetic Improvement , Huazhong Agricultural University;
关键词：
Brassica napus L;;Bacillus thuringienesis;;Cry1C;;Cry2A;;two Bt genes stacking;;agrobacterium-mediated transfor mation;;quality trait
摘  要：
Two Bt genes Cry1 C and Cry2 A were introduced into an elite Brassica napus L. CMS restorer line 7-5 respectively. Two Bt genes from homozygous line with single Bt gene were pyramided to develop two-toxin Brassica napus L. by sexual crossing,insect-resistance and seed quality traits were evaluated. The results showed that all the transgenic plants have improved insect resistance. Enzyme-linked immunosorbent assay(ELISA) indicated that Cry1 C, Cry2 A expressed stably individually and pyramided lines showed a comparable Bt protein level to their single gene parents. The results of the Plutella xylostella two instar larvae inoculated in the laboratory showed that insect resistance of transgenic plants was enhanced Cry1 C transgenic lines cause apparently higher mortality than corresponding two Bt gene plants. Cry1 C lines and two Bt genes pyramiding lines have more enhanced resistance than Cry2 A lines. The pyramiding plants with Cry1 C as female parent showed improved resistance than the plants of Cry2 A as female parent. This suggests Cry1 C plays a maternal effect in Cry1 C ×Cry2A transgenic plants. In glasshouse seedlings evaluation, original 7-5 plants were damaged seriously. All transgenic Brassica napus lines and pyramiding lines exhibit excellent resistance to insect pests. For the seed quality assay, single Bt gene lines and two Bt genes pyramiding transgenic plants are comparable with 7-5 in oil and glucosinate contents.

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