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Position: Home > Articles > Maturation in vitro and Parthenogenetic Activation of Mice Oocytes Animal Husbandry and Feed Science 2011 (12) 12-13+46

小鼠卵细胞体外成熟培养及孤雌激活

作者：

Yuchao Don

单位：

College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu,030801 China

关键词：

oocytes;strontium chloride;mice;ethanol;class;activatio

摘要：

[目的]利用小鼠卵母细胞体外成熟培养及3种激活方法(乙醇激活、氯化锶激活、乙醇—氯化锶激活)对小鼠卵母细胞进行了孤雌激活研究。[方法]小鼠经注射孕马血清促性腺激素(PMSG)48h后,摘取卵巢获得3级未成熟卵母细胞,在成熟培养液(M199100mL+丙酮酸钠2.2mg+双抗100IU/mL+LH2IU/mL+FCS10%)中对小鼠未成熟卵细胞进行体外成熟培养,获得的成熟卵母细胞分别在乙醇、氯化锶、乙醇—氯化锶不同激活剂中激活。[结果]A级卵母细胞成熟率为79%,B级卵母细胞成熟率为70%,C级卵母细胞成熟率为55%。A级卵母细胞使用氯化锶激活率可达80.0%,为最佳方法。[结论]A级卵母细胞成熟培养效果最好。孤雌激活氯化锶激活效果高于乙醇。

译名：

Maturation in vitro and Parthenogenetic Activation of Mice Oocytes

作者：

WANG Hai-dong,XUE Lin-li,YANG Lei,DONG Yan-jun(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)

关键词：

oocytes;maturation in vitro;parthenogenetic activation

摘要：

[Objective]The research aimed to used the maturation in vitro of mice oocytes and three activation methods(ethanol activation,strontium chloride activation,ethanol-strontium chloride activation) and make the parthenogenetic activation study of mice oocytes.[Method]After mice were injected with pregnant mare serum gonadotropin(PMSG) 48 h,ovaries were picked to obtain three classes of immature oocytes.The immature oocytes of mice were in vitro matured and cultured in maturation culture solution(100 mL M199 +2.2 mg sodium pyruvate +100 IU/mL double antibody + 2 IU/mL LH + 10%FCS).The obtained mature oocytes were activated by ethanol,strontium chloride,ethanol-strontium chloride.[Result]The maturation rate of A-class oocytes,B-class oocytes and C-class oocytes were 79%,70% and 55% respectively.The activation rate of A-class oocytes by strontium chloride reached 80.0% and this activation method was the best.[Conclusion]The maturation and culture effects of A-class oocytes were the best.The parthenogenetic activation effect of strontium chloride was higher than that of ethanol.

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小鼠卵细胞体外成熟培养及孤雌激活

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