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Position: Home > Articles > Development of Double Antibody Sandwich ELISA for Detection of Bovine Colostrum Immunoglobulin G FOOD SCIENCE 2016,37 (14) 74-79

牛初乳中免疫球蛋白G双抗夹心ELISA检测方法的建立

作  者:
刘金;王丽威;岳喜庆
单  位:
沈阳农业大学食品学院
关键词:
牛初乳;免疫球蛋白G;单克隆抗体;双抗夹心酶联免疫吸附法
摘  要:
将牛免疫球蛋白G(immunoglobulin G,Ig G)作为免疫原免疫BALB/c小鼠,通过细胞融合、筛选获得分泌抗牛Ig G单克隆抗体的细胞株。制备小鼠腹水抗体,进一步纯化获得抗牛Ig G单克隆抗体。建立双抗夹心酶联免疫吸附法检测牛初乳中Ig G质量浓度,该方法在7.8~1 000 ng/m L范围内有良好的线性关系,最低检出限为7.06 ng/m L,批内变异系数为4.52%,批间变异系数为4.94%,回收率为91.85%~102.45%。此法操作简便、准确度高、稳定性好,可用于实际牛初乳样品的快速检测。
译  名:
Development of Double Antibody Sandwich ELISA for Detection of Bovine Colostrum Immunoglobulin G
作  者:
LIU Jin;WANG Liwei;YUE Xiqing;College of Food Science, Shenyang Agricultural University;College of Science, Liaoning Technology University;
关键词:
bovine colostrum;;immunoglobulin G(Ig G);;monoclonal antibody;;sandwich ELISA
摘  要:
The hybridoma cells secreting monoclonal antibody(Mc Ab) against bovine colostrum immunoglobulin G(Ig G) were developed by cell fusion technology after immunization of BALB/c mice with bovine Ig G. Mc Abs were obtained by purification of mouse ascites. A double antibody sandwich enzyme-linked immunosorbent assay(ELISA) technique was established for the detection of bovine colostrum Ig G. The linear range was 7.8–1 000 ng/m L and the limit of detection(LOD) was 7.06 ng/m L. The intra-assay and inter-assay coefficient of variations were 4.52% and 4.94%, respectively. The recovery rate was 91.85%–102.45%. The ELISA method can provide a simple, accurate and stabile approach for the rapid detection of bovine colostrum Ig G.

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