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Position: Home > Articles > Determination of Polysaccharide Content of Bletilla striata in Ruili,Yunnan by Phenol Sulfuric Acid Colorimetry Chinese Journal of Tropical Agriculture 2018,38 (10) 62-67

苯酚-硫酸比色法测定瑞丽白芨多糖含量

作  者:
李国明;张丽萍;戴余波;张玉龙;李守岭;刘小琼
单  位:
云南省德宏热带农业科学研究所
关键词:
苯酚-硫酸比色法;白芨多糖;含量测定
摘  要:
采用水提醇沉法提取瑞丽白芨粗多糖,以苯酚-硫酸比色法测定白芨多糖含量。结果显示,最大检测波长490 nm, 6%苯酚溶液用量1.2 mL,浓硫酸用量6.5 mL,反应时间60 min,测定结果较好,吸光度与葡萄糖浓度具有良好的线性关系,线性方程为y=0.009x+0.002 3,相关性系数R2=0.999 5。对白芨多糖样品进行加标回收率实验,加标回收率为99.11%,相对标准偏差RSD为0.763 3%,实验准确度和精密度均较好。
译  名:
Determination of Polysaccharide Content of Bletilla striata in Ruili,Yunnan by Phenol Sulfuric Acid Colorimetry
作  者:
LI Guoming;ZHANG Liping;DAI Yubo;ZHANG Yulong;LI Shouling;LIU Xiaoqiong;Institute for Tropical Agriculture Scientific Research of De Hong;
关键词:
phenol sulfuric acid colorimetry;;polysaccharide of Bletilla striata;;content determination
摘  要:
The polysaccharide of Bletilla striata grown in Ruili was extracted by water extraction and alcohol precipitation,and determined by the phenol sulfuric acid colorimetry.The results showed that the polysaccharide was better determined when the maximum wave length was 490 nm,the volume of 6%phenol solution 1.2 mL,the volume of concentrated sulfuric acid 6.5 mL,the reaction time 60 mins.There was a good linear relationship between absorbance and glucose content,the linear equation was y=0.009 x+0.002 3,and the relative standard deviation(RSD) was 0.763 3%.The determination of polysaccharide with this method was better in accurateness and preciseness.

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