作 者：
崔仁勇;王建萍;张克英;丁雪梅;曾秋凤;白世平;罗玉衡
单 位：
四川农业大学动物营养所教育部抗病营养重点实验室
关键词：
蛋黄粉;钒;大鼠;氧化应激;醌氧化还原酶1;核因子E2相关因子2
摘 要：
钒是一种重金属,过量的摄入会造成蛋鸡氧化应激,降低鸡蛋品质并残留到鸡蛋中,影响鸡蛋的安全。本研究主要通过给大鼠饲喂含钒的蛋黄粉,考察其对大鼠生长性能、氧化应激状态及其相关基因表达的影响,为评价钒(有机钒)的生物安全性提供依据。选用27只4周龄雌性Wistar大鼠单笼饲养,设3个处理,每个处理9只,分别在饲粮中添加600 g/kg采食3种钒水平(0、5和10 mg/kg)饲粮的蛋鸡所产鸡蛋制备的蛋黄粉。经实测,3种饲粮分别含钒0.107、0.137和0.164 mg/kg。试验期35 d。结果表明:3个处理大鼠生长性能、器官指数,血浆甘油三酯、丙二醛(MDA)、尿素氮含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,肝脏、肾脏超氧化物歧化酶活性、MDA含量、总抗氧化能力,肝脏ALT、AST活性、谷胱甘肽含量和谷胱甘肽巯基转移酶活性,以及肝脏、肾脏组织结构、肾脏钒残留量均无显著差异(P>0.05)。与处理1相比,处理2、3大鼠肝脏醌氧化还原酶1(NQO1)活性显著下降(P<0.05),NQO1和核因子E2相关因子2 mRNA相对表达量也显著下降(P<0.05)。结果提示:大鼠饲粮中添加600 mg/kg不同钒含量的蛋黄粉对大鼠生长性能和机体氧化还原状态的影响无显著差异,但含钒0.137、0.164 mg/kg可以降低NQO1酶活性并下调抗氧化应答相关基因的表达。
译 名：
Effects of Egg Yolk Powder Including Vanadium on Growth,Oxidative Stress Status and Its Related Gene Expression of Wistar Rats
作 者：
CUI Renyong;WANG Jianping;ZHANG Keying;DING Xuemei;ZENG Qiufeng;BAI Shiping;LUO Yuheng;Key Laboratory of Animal Disease-Resistance of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University;
关键词：
egg yolk powder;;vanadium;;rat;;oxidative stress;;NQO1;;Nrf2
摘 要：
As a kind of heavy metal,excessive intake of vanadium can cause oxidative stress to laying hens,reduce egg quality and make residue in eggs,which affects the safety of eggs. The objective of this study was to explore the effects of feeding egg yolk pow der including vanadium to W istar rats on their growth,oxidative stress status and its related gene expression,and to evaluate the biosecurity of organic vanadium. A total of 27 female W istar rats( 4 weeks old) were allocated to 3 treatments with 9 rats each,and fed three kinds of diets supplemented with 600 g / kg egg yolk pow ders which were prepared with eggs from laying hens fed diets containing 0,5 and 10 mg / kg vanadium,respectively. The three diets actually contained 0. 107,0. 137 and0.164 mg / kg vanadium. The experiment lasted for 35 d. The results showed that there were no significant differences on growth performance,organ indices,contents of plasma triglyceride,malonaldehyde( MDA)and urea nitrogen, activities of plasma alanine aminotransferase( ALT) and aspartate aminotransferase( AST),liver and kidney superoxide dismutase( SOD) activity,MDA content,and total antioxidant capacity( T-AOC),activities of liver ALT and AST,liver glutathione content,and liver glutathione S-transferase activity,as well as histomorphology of liver and kidney and vanadium residual in kidney of rats among 3 treatments( P> 0.05). How ever,compared with treatment 1,liver quinone oxidoreductase 1( NQO1) activity of rats in treatments 2 and 3 was significantly decreased( P < 0. 05),and the relative expression levels of liver NQO1 and nuclear factor erythroid 2-related factor 2( Nrf2) mRNA were significantly decreased( P < 0.05),too. The results indicate that the dietary supplementation of 600 mg / kg egg yolk pow ders with different vanadium content has no significantly different effects on growth performance and oxidation-reduction state of W istar rats. How ever,the NQO1 activity,mRNA expression of NQO1 and Nrf2 are dow n-regulated as the vanadium content increasing( 0. 137 and 0. 164 mg / kg).
