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当前位置: 首页 > 文章 > Variations of phyllosphere microorganisms in asymptomatic and tobacco brown spot leaves before and after spraying 12% difenoconazole + fluxapyroxad SC 农药学学报 2024 (5) 932-948
Position: Home > Articles > 12%苯甲•氟酰胺悬浮剂使用前后未感病和感赤星病烟叶叶际微生物的变化 Chinese Journal of Pesticide Science 2024 (5) 932-948

Variations of phyllosphere microorganisms in asymptomatic and tobacco brown spot leaves before and after spraying 12% difenoconazole + fluxapyroxad SC

作  者：
LI Tong;WANG Hancheng;YE Guo;WANG Qing;NGANGUEM NZALLE Yranney Brice;WANG Feng;CAI Liuti;FENG Ruichao;ZHANG Songbai
单  位：
;College of Agriculture
关键词：
tobacco;brown;spot;DIFENOCONAZOLE;fluxapyroxad;microorganism;communities;diversity;high-throughput;sequencing
摘  要：
12%difenoconazole+fluxapyroxad SC(commercial name:Jiangong)was first released by BASF in China in 2016.It has been registered to control many diseases,including pear scab,apple Alternaria leaf spot,tomato early blight,cucumber powdery mildew,etc.This study evaluated the bioactivity of Jiangong against Alternaria alternata and explored variations of phyllosphere microorganisms in both asymptomatic and tobacco brown spot leaves at different persistence periods(0,5,10,and 15 days post-fungicide application)using high-throughput sequencing technology.The results indicated that Jiangong effectively inhibited mycelial growth(average EC50 value of 0.51μg/mL),conidia germination(average EC50 value of 3.47μg/mL),and the carbon metabolism of A.alternata.Both asymptomatic and symptomatic leaves presented complex microbial communities.Higher fungal diversity was noted in asymptomatic leaves,while higher bacterial diversity was found in symptomatic leaves.After application,the diversity and abundance of microbial community structures in both types of leaves changed over time.Fungal microbiome communities showed greater sensitivity than bacterial groups,with the microbiome communities of asymptomatic leaves being more affected than those of symptomatic leaves.Fungal community diversity decreased for both symptomatic and asymptomatic leaves after 5 days of application,while the diversity of fungal community in symptomatic leaves showed an upward trend after 10 days of application.Meanwhile,bacterial community diversity increased in both symptomatic and asymptomatic leaves after 5 days of application but then declined in asymptomatic leaves after 15 days.The abundance of the dominant function group of phyllosphere bacteria(metabolism,genetic information processing,environmental information processing)was not affected by the application of Jiangong.However,the abundance of the dominant function group of phyllosphere fungi(animal pathogen-endophyte-wood saprotroph,endophyte-plant pathogen,plant pathogen-undefined saprotroph)was significantly affected by the application of Jiangong,and high variation was found in symptomatic leaves than that of asymptomatic leaves.The application of Jiangong-induced alterations in the community structure of the tobacco phyllosphere microbiome provides a basis for future tobacco brown spot control strategies based on phyllospheric microecology.
译  名：
12%苯甲•氟酰胺悬浮剂使用前后未感病和感赤星病烟叶叶际微生物的变化
关键词：
烟草赤星病%苯醚甲环唑%氟唑菌酰胺%微生物群落多样性%高通量测序
摘  要：
12%苯甲?氟酰胺悬浮剂(商品名:健攻),德国巴斯夫公司于2016年在中国首次发布,已被登记用于防治多种病害,包括梨树黑星病、苹果斑点落叶病、番茄早疫病、黄瓜白粉病等.本研究测定了健攻对烟草赤星病菌的抑制活性,并通过高通量测序技术分析了其施用后不同持效期(0、5、10和15 d)未感病和感赤星病烟叶叶际微生态的变化.结果表明:健攻对赤星病菌菌丝生长(平均EC50值为0.51μg/mL)、孢子萌发(平均EC50值为3.47μg/mL)和碳源代谢均有较强的抑制活性.烟草未感病和感赤星病烟叶叶际微生物群落结构复杂,其中感病烟叶叶际真菌多样性低于未感病烟叶,而细菌多样性高于未感病叶片.未感病和发病烟叶叶际微生物多样性和丰度均随药剂施用时间发生变化,叶际真菌群落结构较细菌群落结构敏感,未感病烟叶叶际微生物较感病烟叶受药剂影响大.药后5 d,未感病烟叶和感病烟叶叶际真菌多样性均下降,10 d时感病烟叶叶际真菌多样性上升.药后5 d,感病和未感病烟叶叶际细菌多样性均有所上升,15 d时未感病烟叶叶际细菌多样性下降.施药后,2种烟叶叶际细菌优势功能类群(新陈代谢、遗传信息处理、环境信息处理)的相对丰度未受影响;而叶际真菌优势功能类群(病原菌-内生菌-木质腐生菌、内生菌-植物病原菌、植物病原菌-未定义腐生菌)的相对丰度发生变化,且感病烟叶较未感病烟叶变化大.施用健攻可诱导烟叶叶际微生物群落结构发生改变,该结果可为今后基于叶际微生态的烟草赤星病防治提供参考依据.

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