当前位置: 首页 > 文章 > 苯酚-硫酸法测定人参茎叶多糖的条件研究 安徽农业科学 2012,40 (24) 12023-12025
Position: Home > Articles > Determination of Polysaccharides in Ginseng Stem Leaves by Phenol-Sulfuric Acid Method Journal of Anhui Agricultural Sciences 2012,40 (24) 12023-12025

苯酚-硫酸法测定人参茎叶多糖的条件研究

作  者:
苏晓田;孟凡丽;王雅华
单  位:
辽宁农业职业技术学院生物技术系
关键词:
人参茎叶;苯酚-硫酸法;多糖
摘  要:
[目的]优选苯酚-硫酸法测定人参茎叶多糖含量的因素条件。[方法]采用紫外分光光度法测定人参茎叶多糖的含量,并采用正交试验优选测定的条件。[结果]苯酚-硫酸法测定人参茎叶多糖含量的最佳测定波长为485 nm;苯酚-硫酸法的最佳显色条件为A1B2C1,即:浓度6%苯酚的体积为1.0 ml,浓硫酸的体积为6.0 ml,反应时间为25 min;粗多糖含量在10~70μg/ml之间与吸光度有很好的线性关系,回归方程为Y=0.013 2X-0.085 7,相关系数R为0.999 6;试验方法的回收率为100.36%,RSD为1.26%,显色液在120min内稳定性良好。[结论]该方法准确可靠,为人参茎叶的进一步开发利用提供了依据。
译  名:
Determination of Polysaccharides in Ginseng Stem Leaves by Phenol-Sulfuric Acid Method
作  者:
SU Xiao-tian et al(Department of Biotechnology,Liaoning Agricultural College,Yingkou,Liaoning 115009)
关键词:
Ginseng stem leaves;Phenol-sulfuric acid method;Polysaccharides
摘  要:
[Objective] To optimize the factor conditions of the polysaccharides in ginseng stem leaves by phenol-sulfuric acid method.[Method] Polysaccharides contents in ginseng stem leaves were detected by UV Spectrophotometry;detection condition was optimized by orthogonal test.[Result] The optimal wavelength was 485 nm for the determination of polysaccharides contents in ginseng stem leaves were detected by phenol-sulfuric acid method.The optimal color condition was A1B2C1,which were 1.0 ml of 6% phenol,6.0 ml concentrated sulfuric acid,and 25 min reaction time.Crude polysaccharides had good linear relationship with absorbency within 10-70 μg/ml.The regression equation was Y=0.013 2X-0.085 7;correlation coefficient R was 0.999 6;recovery rate of test method was 100.36%,and RSD was 1.26%.The coloured solution had good stability within 120 min.[Conclusion] This method was accurate and reliable,and provided references for the further development and utilization of ginseng stem leaves.

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