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当前位置: 首页 > 文章 > 谷物中柠檬黄和日落黄高效液相色谱检测方法的优化 安徽农业科学 2012,40 (9) 279-281+546
Position: Home > Articles > Optimization of the Test of Tartrazine and Sunset Yellow in Cereals by Means of HPLC Journal of Anhui Agricultural Sciences 2012,40 (9) 279-281+546

谷物中柠檬黄和日落黄高效液相色谱检测方法的优化

作  者：
初红涛;张晖;王军;靳凤龙;苏立强
单  位：
齐齐哈尔大学;青岛科技大学
关键词：
谷物;柠檬黄;日落黄;高效液相色谱;优化
摘  要：
[目的]探索利用高效液相色谱检测人工色素的优化方法。[方法]以高效液相色谱方法梯度洗脱,分离、分析谷物中的柠檬黄和日落黄,对前处理温度、超声时间、柱温、梯度洗脱条件进行考察,确定最佳检测条件。[结果]在样品前处理温度40℃下,最佳色谱分析条件为流动相为含pH为6.8的醋酸-醋酸铵(0.02 mol/L)的甲醇/水混合液、柱温35℃,梯度洗脱条件甲醇:20%～35%,3%/min;35%～98%,6%/min;98%继续8 min。采用HPLC优化法测定谷物中柠檬黄和日落黄含量的精密度良好,二者平均加样回收率分别为97.9%和100.2%,最低检出限分别为0.02、0.03 mg/kg。[结论]该方法缩短了分析时间,提高了检测灵敏度和人工色素的分离度。
译  名：
Optimization of the Test of Tartrazine and Sunset Yellow in Cereals by Means of HPLC
作  者：
CHU Hong-tao et al(Qiqihar University,Qiqihar,Heilongjiang 161006)
关键词：
Cereals;Tartrazine;Sunset yellow;High performance liquid chromatography;Optimization
摘  要：
[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph.[Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals.The pre-treatment temperature,ultrasonic time,the column temperature and the elution conditions were studied,and the best experimental conditions were confirmed.[Result] At 40 ℃,the optimal chromatography analysis condition was moving phase,with pH(6.8),methanol/water of acetic acid-ammonium acetate(0.02 mol/L),and column temperature(35 ℃).Gradient elution condition was 20%-35% methanol,3%/min;35%-98%,6%/min;98%,8 min.The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC.The average recoveries of standard addition were 97.9% and 100.2%,and the detection limit were 0.02 mg/kg and 0.03 mg/kg.[Conclusion] This method shortened the analysis time,improved the detection sensitivity and the resolution of artificial color.

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