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当前位置: 首页 > 文章 > 棉田Cry1Ac蛋白降解菌株12T-103的分离鉴定及降解能力研究 塔里木大学学报 2018 (4) 59-63
Position: Home > Articles > Isolation and Identification of Cry1Ac Protein Degrading Bacterial Strain 12T-103 and Its Degradation Ability from Cotton Field Soil Journal of Tarim University 2018 (4) 59-63

棉田Cry1Ac蛋白降解菌株12T-103的分离鉴定及降解能力研究

作  者：
肖海兵;董震宇;董红强;符娜;尚北晨;杨明禄
单  位：
塔里木大学植物科学学院
关键词：
Cry1Ac蛋白;棉田土壤;降解细菌;降解能力
摘  要：
为分离鉴定棉田转Cry1Ac基因棉的Cry1Ac蛋白土壤降解细菌及测定其降解能力。采用蛋白质凝胶电泳(SDSPAGE)、酶联免疫法(ELISA)法和常规细菌培养与鉴定技术,对第一师12团棉田土壤中Cry1Ac蛋白降解菌进行分离、纯化、形态生理生化观察以及降解功能测定。结果表明:从棉田土壤分离一株具有降解Cry1Ac蛋白能力的土壤细菌,命名为12T-103菌株,细菌形态学、生理生化特征和16S r DNA序列鉴定为枯草芽孢杆菌(Bacillus subtilis),该菌株对Cry1Ac蛋白的降解效果较为明显,在1h时Cry1Ac蛋白含量由15. 95μg·L-1降至0. 68μg·L-1,其降解程度达极显著水平(p <0. 01),其降解率为92. 26%。结论:分离于转基因棉田的12T-103菌株为枯草芽孢杆菌(Bacillus subtilis),该菌株对Cry1Ac蛋白的降解能力较强。
译  名：
Isolation and Identification of Cry1Ac Protein Degrading Bacterial Strain 12T-103 and Its Degradation Ability from Cotton Field Soil
作  者：
Xiao Haibing;Dong Zhenyu;Dong Hongqiang;Fu Na;Shang Beichen;Yang Minglu;College of Plant Science,Tarim University;
单  位：
Xiao Haibing%Dong Zhenyu%Dong Hongqiang%Fu Na%Shang Beichen%Yang Minglu%College of Plant Science,Tarim University
关键词：
Cry1Ac protein;;cotton soil;;degrading bacteria;;degradation ability
摘  要：
For isolation and identification of Cry1 Ac protein degrading bacterial strain and its degradation ability from the soil of planting Bt cotton in the farm 12,first division of Xinjiang production and construction corps,the Cry1 Ac protein degrading bacterial strain and its bacterial form,physiology and biochemistry in the soil were isolated,purified and detected with SDS-PAGE,enzyme linked immunoassay( ELISA) and conventional bacterial culture and identification technologies. The results showed that one Cry1 Ac protein degrading bacterial strain was isolated and named as 12 T-103,and the 12 T-103 was identified as Bacillus subtilis according to the phenotype observation,characteristics of physiology and biochemistry and 16 S r DNA analysis,which had great significant effect on the degradation of Cry1 Ac protein from 15. 95μg·L-1 to 0. 68μg·L-1 in 1 h( p < 0. 01),and the degradation rate of Cry1 Ac protein was 92. 26%. It indicated that the 12 T-103 from the Bt cotton planting soil was Bacillus subtilis,and had strong degradation ability on Cry1 Ac protein.

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