当前位置: 首页 > 文章 > 鸡催乳素受体基因多态性与就巢及产蛋性状的关联分析 中国农业科学 2013,46 (12) 2558-2565
Position: Home > Articles > 鸡催乳素受体基因多态性与就巢及产蛋性状的关联分析 Scientia Agricultura Sinica 2013,46 (12) 2558-2565

鸡催乳素受体基因多态性与就巢及产蛋性状的关联分析

作  者:
杜晓惠;李丛艳;刘俊莹;牛伟贺;张细权
单  位:
华南农业大学动物科学学院;四川农业大学动物科技学院
关键词:
宁都黄鸡;PRLR基因;多态性;就巢;产蛋性状
摘  要:
[目的]揭示与繁殖性状关系密切的催乳素受体(prolactin receptor,PRLR)基因与中国地方鸡种的就巢性及产蛋性能的关系.[方法]本试验采用PCR-SSCP方法,以576只宁都黄鸡为材料,检测PRLR基因的多态性,并进行多态性与就巢和产蛋性状间的关联分析.[结果]证实了前人研究过的SNP1(T10862C,外显子3)和SNP2(T25670C,外显子6)均与就巢性状无显著关联(P>0.05);SNP2对300 d产蛋量影响显著(P<0.05);新发现的SNP3(G30716A,内含子8)显著影响开产日龄(P<0.05);新发现的SNP4(A31900G,外显子10)极显著地影响就巢率(P<0.01),显著影响就巢天数和300 d产蛋量(P<0.05).由4个SNPs构建的不同单倍型在开产日龄上的差异达到极显著水平(P<0.01),在其它性状上的差异不显著(P>0.05).[结论]PRLR基因的多态性与就巢和产蛋性状相关,SNP4的效应更为突出.
单  位:
1College of Animal Science,Sichuan Agricultural University,Ya'an 625014,Sichuan%2Sichuan Animal Science Academy,Chengdu 610066%3College of Animal Science,South China Agricultural University,Guangzhou 510642
关键词:
Ningdu yellow chicken%PRLR gene%polymorphism%broodiness%egg production traits
摘  要:
[Objective] In order to reveal the relationship between bloodiness and egg reproduction of Chinese indigenous chicken and prolactin receptor(PRLR) gene,which closely related to reproductive traits among amimals.[Method]Using the PCR-SSCP method and 576 Ningdu Yellow chicken,the experiment studied the polymorphism of PRLR gene and its association with nesting and egg production traits.[Result]The results confirmed previous studies that SNP1(T10862C,exon 3) and SNP2(T25670C,exon 6) had no significant association with broody traits(P>0.05).The effect of SNP2 on egg production of 300 d(EN-300 d) was significantly(P<0.05).The new SNP3(G30716A,intron 8) significantly influenced the age at first egg(AFG)(P<0.05).The novel SNP4(A31900G,exon 10) had an extremely remarkable(P<0.01) impact on the nesting rate,significant(P<0.05) impact on the total broody days and EN-300 d.The differences among different haplotypes constructed by the four SNPs,in AFG reached an extremely significant level(P<0.01),in other traits were no significant differences(P>0.05).[Conclusion] PRLR gene polymorphism was associated with broodiness and egg production traits,especially the effect of SNP4.

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