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当前位置: 首页 > 文章 > 酵母β-葡聚糖对脂多糖诱导的绵羊淋巴细胞氧化应激损伤的保护作用 动物营养学报 2020,32 (6) 2904-2910
Position: Home > Articles > Protective Effects of β-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte Chinese Journal of Animal Nutrition 2020,32 (6) 2904-2910

酵母β-葡聚糖对脂多糖诱导的绵羊淋巴细胞氧化应激损伤的保护作用

作  者：
于春微;李冬芳;刘世雄;郝凌魁;高民;胡红莲;刘大程
单  位：
关键词：
酵母β-葡聚糖;淋巴细胞;脂多糖;活性氧;抗氧化
摘  要：
本试验旨在研究酵母β-葡聚糖对绵羊淋巴细胞抗氧化能力的影响。在建立体外脂多糖(LPS)诱导绵羊淋巴细胞氧化应激模型的基础上,通过在培养液中添加不同浓度[0(对照)、5、10、20、50、100μg/mL]的酵母β-葡聚糖,运用四甲基偶氮唑盐(MTT)比色法测定酵母β-葡聚糖对细胞活性的影响,用生物化学法测定酵母β-葡聚糖对绵羊淋巴细胞抗氧化指标变化的影响。结果表明:0～20μg/mL酵母β-葡聚糖作用于淋巴细胞,细胞存活率无显著变化(P>0.05),当使用50μg/mL酵母β-葡聚糖处理淋巴细胞后,细胞存活率显著降低(P<0.05);在LPS作用于淋巴细胞后,与对照组相比较,丙二醛(MDA)含量极显著增多(P<0.01),超氧化物歧化酶(SOD)和氧化氢酶(CAT)活性显著下降(P<0.05),谷胱甘肽过氧化物酶(GSH-Px)活性极显著下降(P<0.01);添加10μg/mL酵母β-葡聚糖作用6 h后,与LPS组相比较,淋巴细胞内SOD和CAT活性极显著增强(P<0.01),GSH-Px活性显著增强(P<0.05),MDA和ROS含量极显著降低(P<0.01)。综上,LPS可诱导绵羊淋巴细胞氧化应激,酵母β-葡聚糖可抑制LPS诱导的淋巴细胞氧化损伤,对LPS诱导的绵羊淋巴细胞氧化损伤有良好的保护作用,存在剂量依赖效应。
译  名：
Protective Effects of β-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte
作  者：
YU Chunwei;LI Dongfang;LIU Shixiong;HAO Lingkui;GAO Min;HU Honglian;LIU Dacheng;College of Veterinary Medicine,Inner Mongolia Agricultural University;Research Institute of Animal Nutrition and Feed,Inner Mongolia Academy of Animal Sciences;
关键词：
yeastβ-glucan;;lymphocyte;;lipopolysaccharide;;reactive oxygen species;;antioxidant
摘  要：
This experiment was conducted to study the effects ofβ-glucan from Saccharomyces cerevisiae on the antioxidant capacity of sheep lymphocytes. Based on the establishment of lipopolysaccharide(LPS)-induced oxidative stress in sheep lymphocytes,the effects ofβ-glucan on cell viability were determined by MTT assay by adding different concentrations[0(control),5,10,20,50 and 100μg/mL]ofβ-glucan to the culture medium. The biochemical method was used to determine the effects ofβ-glucan on the changes of antioxidant indexes in sheep lymphocytes. The results showed as follows: β-glucan showed no cytotoxicity after being applied to lymphocytes at a concentration of 0 to 20μg/mL,when the concentration ofβ-glucan was 50μg/mL,the cell viability decreased significantly(P<0.05);compared with the control group,the malonaldehyde(MDA)content extremely significantly increased(P<0.01),superoxide dismutase(ROS)and catalase(CAT)activities extremely significantly decreased(P<0.05),and glutathion peroxidase(GSH-Px)activity decreased extremely significantly(P<0.01)in lymphocytes treated by LPS. After adding 10μg/mLβ-glucan for 6 h,compared with the LPS group,the activity of SOD and CAT in lymphocytes extremely significantly increased(P<0.01),the activity of GSH-Px significantly increased(P<0.05),and the contents of MDA and ROS extremely significantly decreased(P<0.01). In conclusions,LPS can induce oxidative stress in sheep lymphocytes.β-glucan can inhibit LPS-induced oxidative injury of lymphocytes and has a good protective effect on LPS-induced oxidative injury of sheep lymphocytes,and there is a dose-dependent effect.

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