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当前位置: 首页 > 文章 > 不同浓度葡萄糖、NaCl和KCl对蛇鮈精子活力的影响 安徽农业大学学报 2012,39 (6) 41-44
Position: Home > Articles > Effects of different concentrations of glucose,NaCl and KCl on the sperm motility in Saurogobio dabryi Journal of Anhui Agricultural University 2012,39 (6) 41-44

不同浓度葡萄糖、NaCl和KCl对蛇鮈精子活力的影响

作  者：
丁淑荃;李飞;万全;许进进
单  位：
安徽农业大学动物科技学院;浙江省淡水水产研究所
关键词：
蛇鮈;溶质;浓度;精子活力
摘  要：
在光学显微镜下,研究不同浓度的葡萄糖、NaCl和KCl对蛇鮈精子活力的影响,为蛇鮈精子保存液和人工授精时精卵结合液的组成提供科学依据,为蛇鮈的人工繁育奠定基础。结果表明,葡萄糖溶液浓度为125mmol·L-1时,精子快速运动时间最长,为37.87±0.99 s,精子寿命亦最长,为186.78±32.65 s;NaCl浓度为68 mmol·L-1时,精子快速运动时间最长,为49.25±3.25 s,NaCl浓度为51 mmol·L-1时,精子的寿命最长为153.41±30.84 s;KCl溶液的最适浓度为125 mmol·L-1,精子的快速运动时间为36.49±4.18 s,而KCl溶液的浓度为75 mmol·L-1时精子的寿命最长为664.03±155.09 s。
译  名：
Effects of different concentrations of glucose,NaCl and KCl on the sperm motility in Saurogobio dabryi
作  者：
DING Shu-quan1,LI Fei2,WAN Quan1,XU Jin-jin1(1.School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036; 2.Zhejiang Institute of Freshwater Fisheries,Huzhou 313001)
关键词：
Saurogobio dabryi;solute;concentration;sperm motility
摘  要：
In this paper,the effects of different concentrations of glucose,NaCl and KCl on the sperm motil-ity of Saurogobio dabryi were studied under light microscope to supply scientific evidence for the composition of sperm preservation solution and fertilization solution for sperm and eggs,and to set a foundation for the artificial propagation of Saurogobio dabryi.The results showed that when the concentration of glucose was 125 mmol/L,the sperm had the longest fast moving time as 37.87 ± 0.99 s,and the longest sperm lifetime as 186.78 ± 32.65 s;when the concentration of NaCl was 68 mmol·L-1,the sperm had the longest fast moving time as 49.25±3.25 s;when the concentration of NaCl was 51 mmol·L-1,the sperm had the longest sperm lifetime as 153.41±30.84 s;when the concentration of KCl was 125 mmol·L-1,the sperm had the longest fast moving time,as 36.49±4.18 s;when the concentration of KCl was 75 mmol·L-1,the sperm had the longest sperm lifetime as 664.03±155.09 s.

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