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当前位置: 首页 > 文章 > 围产期蒙古牛外周中性粒细胞凋亡相关基因的表达分析 河南农业科学 2018 (6) 127-133
Position: Home > Articles > Apoptotic Gene Expression of Peripheral Blood Neutrophils in Perinatal Mongolia Cattle Journal of Henan Agricultural Sciences 2018 (6) 127-133

围产期蒙古牛外周中性粒细胞凋亡相关基因的表达分析

作  者：
刘阳;张新颖;耿万友;毛景东;杜立银
关键词：
凋亡基因;基因表达特征;中性粒细胞;围产期;蒙古牛
摘  要：
选择3头2~3胎次妊娠蒙古牛,分别于分娩前后以及分娩当天采集静脉血,分离中性粒细胞(PMNs)、血清,采用RT-PCR、Western blotting、放射免疫法、全自动动物血液分析仪检测了围产期蒙古牛外周PMNs凋亡相关基因Bcl-2、Bax、P53、Cyt-c在转录和蛋白质水平上的变化,外周血主要白细胞数量,血清中雌二醇(E2)、孕酮(P4)及糖皮质激素(GC)的浓度变化,以揭示蒙古牛围产期免疫抑制下外周PMNs的免疫状态,从而为PMNs免疫抑制机制研究提供理论参考。结果显示,外周血PMNs和单核细胞(MC)总数及淋巴细胞数量总体上均以分娩当天为拐点呈先降后升的趋势。外周血血清E2质量浓度于分娩前22 d逐渐升高,分娩前2 d升至最高,而后下降;P4质量浓度于分娩前22 d开始逐渐下降,分娩当天迅速下降,之后趋于平缓;GC质量浓度变化趋势与P4相反。凋亡抑制基因Bcl-2 mRNA和蛋白表达量均以分娩当天为拐点呈先升后降的趋势,促凋亡基因Bax、P53和Cyt-c mRNA和蛋白表达趋势均与Bcl-2相反。总之,围产期蒙古牛外周PMNs凋亡相关基因表达呈明显动态变化趋势,临近分娩时凋亡抑制作用显著增强,分娩时抑制效应达到峰值,分娩后降低。
译  名：
Apoptotic Gene Expression of Peripheral Blood Neutrophils in Perinatal Mongolia Cattle
作  者：
LIU Yang;ZHANG Xinying;GENG Wanyou;MAO Jingdong;DU Liyin;College of Veterinary Medicine,Inner Mongolia Agricultural University;College of Animal Science and Technology,Inner Mongolia University for Nationalities;
单  位：
LIU Yang%ZHANG Xinying%GENG Wanyou%MAO Jingdong%DU Liyin%College of Veterinary Medicine,Inner Mongolia Agricultural University%College of Animal Science and Technology,Inner Mongolia University for Nationalities
关键词：
Apoptotic gene;;Characteristics of gene expression;;PMNs;;Perinatal period;;Mongolia cattle
摘  要：
The blood samples were collected before,during and after childbirth from three pregnancy Mongolian cows giving birth two or three times,and the peripheral polymorphonuclear neutrophils( PMNs),sera were separated for detecting the level of transcription and protein expression of apoptosis related genes,including Bcl-2,Bax,P53,and Cyt-c in peripheral PMNs using RT-PCR,and Western blotting. The changes of peripheral neutrophils number,and concentration changes of blood serum estradiol( E2),progesterone( P4) and glucocorticoids( GC) were detected for revealing peripheral PMNs immune state of the Mongolian cow in the perinatal immune suppression,so as to provide theoretical reference for the study of PMNs immunosuppression mechanism. The results indicated that the total number of PMNs,mononuclear cells( MCs),and lymphocytes in peripheral blood showed down first and then up the childbirth to be the inflection point. The E2 mass concentration gradually increased 22 daysbefore delivery,and reached a peak 2 days before delivery. The mass concentration of P4 decreased gradually 22 days before delivery,rapidly decreased on the day of delivery and then leveled off. GC mass concentration trend was opposite to that of P4. The expression of Bcl-2 showed a trend of rising first and then falling at the inflection point on the day of childbirth. The expression trend of apoptosis promoting genes Bax,P53 and Cyt-c was opposite to that of Bcl-2. In short,the apoptosis related gene expression showed a trend of obvious dynamic changes and near to childbirth the apoptosis inhibition significantly enhanced,reaching a peak during childbirth,reducing after childbirth.

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