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Position: Home > Articles > Study on Purification of Polyphenoloxidase in Flesh of Nanguo Pear by Ammonium Sulphate FOOD SCIENCE 2005,26 (7) 32-34

硫酸铵纯化南果梨果肉多酚氧化酶的研究

作  者:
辛广;朱凤妹;刘长江
单  位:
鞍山师范学院化学系;沈阳农业大学食品学院
关键词:
南果梨;多酚氧化酶;硫酸铵;纯化;同工酶
摘  要:
南果梨果肉中多酚氧化酶经硫酸铵分级沉淀、透析后进行了纯化,比活力提高2.1倍;根据凝胶电泳对其同工酶进行分析,发现其粗酶液中有2条谱带,Rf值分别为0.21、0.26。经35%~85%硫酸铵沉淀透析液纯化透析后同功酶共有5条谱带,Rf值分别为0.21、0.26、0.30、0.39、0.48和0.51。
译  名:
Study on Purification of Polyphenoloxidase in Flesh of Nanguo Pear by Ammonium Sulphate
作  者:
XIN Guang1,ZHU Feng-mei2,LIU Chang-jiang2(1.Department of Chemistry, Anshan Normal University, Anshan 114005, China;2.Food Science College, Shenyang Agricultural University, Shenyang 110161, China)
关键词:
Nanguo pear;PPO;ammonium sulphate;purification;isoenzyme
摘  要:
The purification of polyphenoloxidase(PPO) in flesh of Nanguo pear has been studied. At the end of the (NH4)2SO4precipitation and dialysis step, 2.1-fold purification was achieved. According to the gel electrophoresis analysis, two bands ofisoenzymes of crude polyphenoloxidase in flesh were expressed and Rf value respectively, 0.21, 0.26 and six bands ofpolyphenol oxidase in flesh after the (NH4)2SO4 precipitation were expressed and Rf value respectively, 0.21, 0.26, 0.30,0.39, 0.48 and 0.51.

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