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当前位置: 首页 > 文章 > 拟南芥耐硒突变体的鉴定及基因定位 湖北农业科学 2018 (2) 115-118
Position: Home > Articles > Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana Hubei Agricultural Sciences 2018 (2) 115-118

拟南芥耐硒突变体的鉴定及基因定位

作  者：
陈大清;钟育海;李应生;苏爱国;朱云芬;李亚男
单  位：
长江大学发展规划处;仲恺农业工程学院农业与生物学院;恩施州种子管理局;长江大学生命科学学院;恩施州硒应用技术与产品开发研究院
关键词：
拟南芥(Arabidopsis thaliana);硒盐胁迫;突变体;基因定位
摘  要：
在硒盐胁迫下通过根弯曲特性筛选获得拟南芥(Arabidopsis thaliana)耐硒突变体,并以突变体为母本与野生型(Ler)杂交获得基因型完全杂合的F1代;自交产生的F2代硒敏感,硒耐受的分离比为3∶1,表明该突变体是隐性单基因遗传。F2代选出132株具有耐硒特性的植株作为图位克隆的群体,从应用SSLP和In/Del分子标记进行基因定位,通过分析目的基因与各分子标记之间的连锁关系,发现耐硒突变体基因位于第I号染色体F12P19和NGA111的分子标记之间。
译  名：
Idengtification and Lcation of the Selenium Resistant Gene in Arabidopsis thaliana
作  者：
CHEN Da-qing;ZHONG Yu-hai;LI Ying-sheng;SU Ai-guo;ZHU Yun-fen;LI Ya-nan;College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering;Enshi Seed Authority;School of Life Science,Yangtze University;Enshi Institute of Selenium Application Technology and Product Development;Development and Planning Department,Yangtze University;
关键词：
Arabidopsis thaliana;;selenate stress;;mutant;;gene lacation
摘  要：
A selenate-torlerance arabidopsis mutant was obtained by using the root-bending trait under selenate stress. It was conformed that the selenate resistant characteristics could be steady inheritance. Then this mutant was crossed with the wildtype Ler. and F1 generation were created with heterozygous type completely. The F2 crossed by theirself were expressed the separation ratio of 3∶1 in response of sensitive selenium to resistent selenium,It were suggested that this mutant was a monogenic recessive mutation. Map-based cloning population were composed of the 132 selenate-tolerance seedlings in F2 generation. Using SSLP and In/Del based on PCR to preliminary chromosomal location. Through linkage analysis,it's indicated that this mutant gene was located on the chromosome I,which was mapped between molecular marker F12 P19 and NGA111.

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