单 位：
上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心
关键词：
草鱼皮;蛋白酶;酶解;胶原蛋白肽;嗜热链球菌
摘 要：
嗜热链球菌被认为是一种多功能益生菌,将Neutrase 0.8L、Protease P"Amano"6、Prote AX、Alcalase 2.4L FG和风味中性蛋白酶5种草鱼皮蛋白水解物作为培养嗜热链球菌的氮源,以水解物对嗜热链球菌的增殖效果为评价指标,选择最佳水解用酶,并利用正交试验优化酶解条件。结果表明,5种微生物蛋白酶中,Neutrase 0.8L是水解草鱼皮蛋白的最佳用酶,最佳的酶解条件为:加酶量1%、酶解时间80 min、起始p H 6.5、料水比1∶2(m/V)。利用Neutrase 0.8L酶解法制备得到的富含胶原蛋白肽的草鱼皮蛋白水解物,是一种培养嗜热链球菌的理想氮源。
译 名：
Enzymatic Preparation of Protein Hydrolysate from Grass Carp Skin for Use as a Nitrogen Source for Streptococcus thermophilus Growth
作 者：
CHEN Jiankang;BAO Jianqiang;Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, College of Food Science and Technology,Shanghai Ocean University;
关键词：
grass carp skin;;protease;;hydrolysis;;collagen-derived peptide;;Streptococcus thermophilus
摘 要：
Streptococcus thermophilus is regarded as a multi-functional probiotic bacterium. Five protein hydrolysates from grass carp skin were prepared using Neutrase 0.8L, Protease P "Amano" 6, Prote AX, Alcalase 2.4L FG and Flavourzyme, respectively, for use as nitrogen sources to culture Streptococcus thermophilus. Among the five proteases, Neutrase 0.8L was found to be the best enzyme to produce the optimal nitrogen source for the growth of Streptococcus thermophilus. In further investigations, we optimized hydrolysis conditions using orthogonal array experiments. The results showed that the optimal hydrolysis conditions were as follows: enzyme dosage, 1%; hydrolysis time, 80 min; initial p H, 6.5; and ratio between fresh skin and water, 1:2(m/V). The hydrolysate obtained under these conditions was rich in collagen-derived peptides as a new optimum nitrogen source for Streptococcus thermophilus incubation.
