作 者：
吴晓洒;蔡路昀;黄作庆;苏洵;冷利萍;宋卓;励建荣
单 位：
渤海大学食品科学与工程学院;大连天宝绿色食品股份有限公司
摘 要：
以草鱼皮为原料,采用六种蛋白酶酶解,以DPPH自由基清除率和水解度为指标,筛选两种较优的酶,再通过响应面分别优化两种酶制备抗氧化酶解液的工艺条件,并在此基础上进行双酶酶解实验,确定酶解草鱼皮的最优工艺。结果表明:碱性蛋白酶酶解草鱼皮的最适条件为加酶量6.3%、料水比1∶40.6、温度51.3℃、时间115.1 min,此时清除率最高为54.98%。木瓜蛋白酶最适条件为加酶量4.6%、料水比1∶41.2、温度50.5℃、时间133.0 min,清除率最高为41.06%。采用分步酶解(先碱性蛋白酶后木瓜蛋白酶)得到的酶解液DPPH自由基清除能力最高为92.07%,明显优于单酶水解和混合酶解的效果。
译 名：
Optimization of enzymolysis conditions for antioxidant components from Grass Carp Skin by response surface methodology
作 者：
WU Xiao-sa;CAI Lu-yun;HUANG Zuo-qing;SU Xun;LENG Li-ping;SONG Zhuo;LI Jian-rong;College of Food Science and Engineering of Bohai University,National & Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,Food Safety Key Lab of Liaoning Province;College of Food Science,Southwest University;Da Lian Tian Bao Green Foods Co.,Ltd;
关键词：
Grass carp skin;;enzymatic hydrolysis;;response surface methodology;;extraction;;antioxidant activity
摘 要：
Six kinds of proteases were used to hydrolyze grass carp skin in order to select two optimum proteases by determining DPPH radical scavenging capacity and degree of hydrolysis( DH).Response surface methodology( RSM) was used to optimize hydrolysis conditions of two optimum proteases.Based on the results of RSM,the double enzyme hydrolysis tests were carried out to find out the best hydrolysis conditions.The results showed that hydrolysis conditions for alcalase were enzyme addition amount of 6.3%,material- liquid ratio of 1 ∶40.6,hydrolysis temperature of 51.3 ℃,and hydrolysis time of 115.1 min,and the scavenging activity on DPPH free radicals under the above conditions reached 54.98%.The optimal hydrolysis conditions for papain were 4.6%,1 ∶41.2,50.5 ℃ and133.0 min,and the scavenging activity reached 41.06%. By combined and sequential use of alcalase followed by papain,the DPPH scavenging activity was 92.07%,which was more effective than either of them.
