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Position: Home > Articles > Preliminary study on identification of watermelon rhizosphere bacterium JK-3 and its antagonism Acta Agriculturae Shanghai 2011,27 (1) 74-78

西瓜根际细菌JK-3的鉴定及其拮抗性的初步研究

作  者:
陈文俊;曾蓉;陆金萍;宋荣浩;童蕴慧;戴富明
单  位:
扬州大学植物保护系;上海市农业科学院植物保护研究所
关键词:
根际细菌;鉴定;拮抗性;多粘类芽孢杆菌
摘  要:
JK-3菌株是从西瓜根际筛选得到的一种革兰氏阳性菌,大小为3.0~4.2μm×0.7~1.0μm,菌落呈淡黄色、圆形、隆起、无褶皱、边缘整齐。扫描电镜观察表明,该菌周生鞭毛,卵圆形芽孢,孢囊膨大。培养4d后菌落表面有较多粘性物质生成。通过16S rDNA测序、常规生理生化方法以及Biolog微生物自动鉴定系统的碳源测定等3种方法鉴定出该根际细菌为多粘类芽孢杆菌。平板对峙培养和孢子萌发试验表明,该菌株对多种植物病原真菌具有良好的拮抗作用并能产生抗生物质。
译  名:
Preliminary study on identification of watermelon rhizosphere bacterium JK-3 and its antagonism
作  者:
CHEN Wen-jun~(1,2),ZENG Rong~1,LU Jin-ping~1,SONG Rong-hao~1, TONG Yun-hui~2,DAI Fu-ming~(1*) (1 Plant Protection Research Institute of SAAS,Shanghai Key Lab of Protected Horticultural Technology,Shanghai 201106,China;2 Department of Plant Protection,Yangzhou University, Yangzhou 225009,China)
关键词:
Rhizosphere bacterium;;Identification;;Antagonism;;Paenibacillus polymyxa
摘  要:
The strain JK-3 screened from watermelon roots is a kind of Gram-positive bacterium with rod-shape(3.0~4.2μm×0.7~1.0μm),and the colony assumes light yellow and an appearance of round,bulge,no wrinkle and regular edge.Under a scanning electron microscope the bacterium is peritrichous and has an oval spore and a swelling cyst.Cultured for 4 days,the colony produces much sticky substance on its surface.The JK-3 is identified as Paenibacillus polymyxa by three methods-16S rDNA sequencing,conventional physiological and biochemical analysis and the Biolog automated microbes identification system.The dual culture and spore germination tests showed that the JK-3 has high antagonism to many important phytopathogens and can produce antibiotic substance.

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