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当前位置: 首页 > 文章 > 超高效液相色谱法测定水体和沉积物中4种硝基呋喃类抗生素 食品科学 2016,37 (16) 249-253
Position: Home > Articles > Determination of Four Nitrofurans in Water and Sediment Using Ultra Performance Liquid Chromatography FOOD SCIENCE 2016,37 (16) 249-253

超高效液相色谱法测定水体和沉积物中4种硝基呋喃类抗生素

作  者：
王强;王旭峰;赵东豪;黄珂;黎智广;李永贤;杨宏亮;李刘冬
单  位：
中国水产科学研究院南海水产研究所农业部水产品加工重点实验室农业部水产品贮藏保鲜质量安全风险评估实验室
关键词：
超高效液相色谱;水体;沉积物;硝基呋喃
摘  要：
建立超高效液相色谱法同时测定水体和沉积物中的呋喃妥因、呋喃西林、呋喃它酮和呋喃唑酮4种硝基呋喃类抗生素。水体样品过滤后直接用混合型阳离子交换(mixed-mode cation exchange,MCX)固相萃取柱富集净化;沉积物样品经乙腈-0.1%甲酸溶液(8∶2,V/V)提取,MCX固相萃取柱净化。采用BEH C18(100 mm×2.1 mm,1.7μm)色谱柱分离,以0.1%甲酸溶液-乙腈为流动相梯度洗脱。4种硝基呋喃类药物在10.0~200μg/L质量浓度内范围线性关系良好,相关系数R2均大于0.999。水体和沉积物中的加标回收率分别为81.5%~103.2%和73.3%~91.9%,相对标准偏差分别为2.0%~5.8%和3.4%~9.6%(n=5),检出限分别为0.03μg/L和0.6μg/kg,定量限分别为0.1μg/L和2.0μg/kg。该方法可应用于水体和沉积物中硝基呋喃类抗生素的残留检测。
译  名：
Determination of Four Nitrofurans in Water and Sediment Using Ultra Performance Liquid Chromatography
作  者：
WANG Qiang;WANG Xufeng;ZHAO Donghao;HUANG Ke;LI Zhiguang;LI Yongxian;YANG Hongliang;LI Liudong;South China Sea Fisheries Research Institute, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture,Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture,Chinese Academy of Fishery Sciences;
关键词：
ultra performance liquid chromatography(UPLC);;water;;sediment;;nitrofuran
摘  要：
An ultra performance liquid chromatography(UPLC) method was developed for the simultaneous determination of 4 nitrofuran antibiotics(furazolidone, furaltadone, nitrofurazone and nitrofurantion) in water and sediment. Water samples were filtrated and cleaned up using mixed-mode cation exchange solid-phase extraction column(MCX). Sediment samples were extracted with acetonitrile-0.1% formic acid solution(8:2, V/V), and then cleaned up also with MCX solidphase extraction column. The analytes were separated on a BEH C18 column(100 mm × 2.1 mm, 1.7 μm) utilizing gradient elution with a mixture of acetonitrile and 0.1% formic acid solution as the mobile phase. The method showed good linearity in the range of 10.0–200 μg/L with correlation coefficients(R2) all above 0.999. The recoveries were 81.5%–103.2% and 73.3%–91.9% with relative standard deviations(RSDs, n = 5) of 2.0%–5.8% and 3.4%–9.6% for 4 nitrofurans in water and sediment, respectively. The limits of detection(LODs) were 0.03 μg/L and 0.6 μg/kg and the limits of quantitation(LOQ) were 0.1 μg/L and 2.0 μg/kg, respectively. The method can be applied to the determination of nitrofuran antibiotics residues in water and sediment.

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