当前位置: 首页 > 文章 > 利用卡那霉素筛选烟草转基因植株的技术研究 河南农业科学 2008,37 (4) 42-44
Position: Home > Articles > Application of Kanamycin in the Screening of Transgenic Tobacco Journal of Henan Agricultural Sciences 2008,37 (4) 42-44

利用卡那霉素筛选烟草转基因植株的技术研究

作  者:
王永江;张振臣;李洪连;乔奇;张德胜;陈冉
单  位:
河南省农业科学院植物保护研究所;河南农业大学植物保护学院
关键词:
卡那霉素;喷施;筛选;转基因烟草
摘  要:
待供试材料转基因和非转基因云烟87烟株长至5~7片叶时,在第3片叶上连续5 d喷施400 mg/L的卡那霉素溶液,喷后2 d观察,结果非转基因云烟87烟株叶片全部产生黄色斑点,而带有卡那霉素抗性标记基因的转基因云烟87烟草叶片表现正常。利用此方法对转基因烟草T2代3个株系进行筛选,筛选出22株转基因烟草植株,并用PCR方法对此方法的准确性进行了验证,结果表明,此方法对转基因烟草是一种简单快速的筛选方法。
译  名:
Application of Kanamycin in the Screening of Transgenic Tobacco
作  者:
WANG Yong-jiang1,2,ZHANG Zhen-chen2*,LI Hong-lian1,QIAO Qi2,ZHANG De-sheng2,CHEN Ran2(1.College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China;2.Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
关键词:
Kanamycin;Spraying;Screening;Transgenic tobacco
摘  要:
By spraying 400mg/L kanamycin solution for five days on the third leaf of tobacco Yunyan87 in the 5-7 leaf seedlings,all of the leaves of non-transgenic tobacco plants presented the yellow spots in two days later,whereas the leaves of transgenic tobacco plants with resistant kanamycin(Kanr)marker gene were normal.Twenty-two transgenic plants was selected from three transgenic lines by this method.This method was further validated by PCR detection.The result indicated that this method is a simple and efficient method for screening the transgenic tobacco in field.

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