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当前位置: 首页 > 文章 > 茶叶中茶多酚和生物碱的测定及聚类和线性判别分析 食品科学 2010,31 (22) 343-348
Position: Home > Articles > Determination of Polyphenols and Alkaloids in Tea and Cluster-Linear Discrimination Analysis FOOD SCIENCE 2010,31 (22) 343-348

茶叶中茶多酚和生物碱的测定及聚类和线性判别分析

作  者：
肖俊松;袁英髦;张爱雪;曹雁平
单  位：
北京工商大学化学与环境工程学院
关键词：
茶多酚;咖啡碱;茶叶;高效液相色谱;聚类分析;线性判别分析
摘  要：
目的:建立一种反相高效液相色谱方法,测定绿茶、乌龙茶、红茶、白茶和普洱茶中儿茶素[(+)-catechin,C]、表儿茶素[(-)-epicatechin,EC]、表儿茶素没食子酸脂[(-)-epicatechin gallate,ECG]、表没食子儿茶素[(-)-epigallocatechin,EGC]、表没食子儿茶素没食子酸脂[(-)-epigallocatechin gallate,EGCG]、没食子酸(gallic acid,GA)、咖啡因(caffeine,CAF)、可可碱(theobromine,THEO)的水平。以这8种组分为指标对茶叶进行聚类分析和线性判别分析,建立区分绿茶、红茶和乌龙茶的方法。方法:茶叶提取后采用HPLC法测定儿茶素和生物碱含量,色谱柱为C18柱,流动相由甲醇(A)、2%的乙酸(B)等度洗脱,A、B相的体积比为25:75,流速为1mL/min,柱温30℃。采用PDA检测器在278nm检测,参考波长为210nm。采用SPSS14.0对实验数据进行了聚类分析和线性判别分析。结果:在选择的分析条件下,样品中的8种组分获得了理想分离,加标回收率在87%～112.8%之间,并采用外标法对8种组分进行定量。以这8种组分的含量为指标,聚类分析和线性判别分析能对39种茶叶样品进较好的区分。
译  名：
Determination of Polyphenols and Alkaloids in Tea and Cluster-Linear Discrimination Analysis
作  者：
XIAO Jun-song1,2,YUAN Ying-hao1,ZHANG Ai-xue1,CAO Yan-ping1,2,* (1. School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100048, China; 2. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing 100048, China)
关键词：
tea polyphenol;caffeine;tea;high performance liquid chromatography (HPLC);cluster analysis;linear discrimination analysis
摘  要：
Objective: To establish a HPLC method for the determination of (+)-catechin, (-)-epicatechin (EC), (-)-epicatechin gallate (ECG), (-)-epigallocatechin (EGC), (-)-epigallocatechin gallate (EGCG), gallic acid (GA), caffeine (CAF) and theobromine (THEO) in green, oolong and black tea samples, and to develop a discrimination method for green, oolong and black tea by cluster analysis and linear discrimination analysis on the basis of determined chemical components. Methods: Tea samples were extracted by 70% aqueous methanol. Totally 200 mg of grinded tea samples was extracted with 5 mL of 70% aqueous methanol for 10 min in 70 ℃water bath. The extract was centrifuged at 3500 r/min for 10 min, and the supernatant was collected. Then the precipitate was extracted with 5 mL of 70% methanol under the same conditions. The supernatants were combined in a volumetric flask and diluted to 10 mL. A total of 2 mL of such solution was diluted by 5 times with stabilizing solution, filtrated with 0.45 μm membrane, and then used for HPLC analysis. Eight compounds were separated by a C18 column using a mobile phase containing methanol-2% aqueous acetic acid at a ratio of 25:75 (V/V) in an isocratic elution at 30 ℃, and detected using a photodiode array detector at 278 nm with the reference wavelength at 210 nm. The cluster and linear discrimination analyses were performed using SPSS 14.0 software. Results: Eight compounds in all tea samples were well separated under the proposed conditions. The recovery rates of spiked samples were in the range of 87%-112.8%. The contents of 8 compounds determined by an external standard method were used to conduct cluster analysis and linear discrimination analysis. The good differentiation of green, oolong and black tea samples were achieved. Conclusion: This established method is feasible to discriminate green, oolong and black tea.

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